Seki K, Ogasawara M, Sakurada J, Murai M, Masuda S
Department of Bacteriology, Jikei University School of Medicine, Tokyo.
Microbiol Immunol. 1989;33(12):981-90. doi: 10.1111/j.1348-0421.1989.tb03156.x.
The virulence of a pleiotropic Staphylococcus aureus mutant with an extremely low producibility of coagulase and other factors was investigated in mouse. A mutant strain, designated as CL-1, showed the same LD50 and the same intrarenal proliferation as its parental strain, when the mutant organisms were inoculated in mice in high doses. The mutant organisms, however, showed a diminished intrarenal proliferation compared with its parental organisms in low doses. This mutant strain expressed a pleiotropic phenotype such as a concomitant reduction in the producibility of coagulase, alpha-toxin, and Panton-Valentine leucocidin. The total effect due to the reduction in producibility of various factors on the virulence of the mutant strain was investigated with studies on the bacterial resistance to the phagocytic activity of leucocytes. A possible role of coagulase and that of some other staphylococcal exoproteins in the pathogenesis of S. aureus were discussed.
在小鼠中研究了一种多效性金黄色葡萄球菌突变体的毒力，该突变体产生凝固酶和其他因子的能力极低。当以高剂量将突变体菌株接种到小鼠体内时，一种命名为CL-1的突变体菌株与其亲代菌株表现出相同的半数致死剂量（LD50）和相同的肾内增殖情况。然而，当以低剂量接种时，与亲代菌株相比，突变体菌株的肾内增殖有所减少。该突变体菌株表现出多效性表型，如凝固酶、α-毒素和杀白细胞素的产生量同时减少。通过研究细菌对白细胞吞噬活性的抗性，探讨了各种因子产生量减少对突变体菌株毒力的总体影响。讨论了凝固酶和其他一些葡萄球菌外毒素在金黄色葡萄球菌发病机制中的可能作用。
