白天补充二氧化碳对室外培养的武装栅藻生产力和生化组成的影响
Effect of daytime CO2 supplement on productivity and biochemical composition of Scenedesmus armatus under outdoor cultivation.
作者信息
Ritcharoen Watadta, Powtongsook Sorawit, Kangvansaichol Kunn, Pavasant Prasert
机构信息
a Chemical Engineering Research Unit for Value Adding of Bioresources, Department of Chemical Engineering , Faculty of Engineering, Chulalongkorn University , Bangkok , Thailand.
b National Center for Genetic Engineering and Biotechnology , National Science and Technology Development Agency , Phathum Thani , Thailand.
出版信息
Prep Biochem Biotechnol. 2016;46(3):267-73. doi: 10.1080/10826068.2015.1015569.
The cultivation of Scenedesmus armatus was carried out under outdoor Thailand climate conditions. The highest actual growth rate occurred at around 9:00 a.m. to 3:00 p.m., with a wide pH range of 6.4 to 11. The supply of CO2 had slight influence on growth characteristics but did exert some observable effects on nutritional accumulations. Adding CO2 from 2 to 15% by volume in the aeration (0.2 vvm) caused an increase in lipid and protein from 19.8 to 25.6 and 37.8 to 48.2% w/w, respectively, whereas carbohydrate decreased from 42.5 to 26.2% w/w. Scenedesmus armatus cultivated with 2% CO2-enriched air provided the highest the average of the average biomass productivity of 91.25 mg L(-1) d(-1), which corresponded to a CO2 fixation of 165 mg CO2 L(-1) d(-1) with the average lipid, protein, and carbohydrate productivities of 22.24, 38.34, and 30.67 mg L(-1) d(-1).
扁形栅藻的培养是在泰国室外气候条件下进行的。最高实际生长速率出现在上午9点至下午3点左右，pH值范围较宽，为6.4至11。二氧化碳的供应对生长特性影响较小，但对营养物质积累有一些明显影响。在曝气（0.2 vvm）中添加体积分数为2%至15%的二氧化碳，导致脂质和蛋白质分别从19.8%（w/w）增加到25.6%（w/w）以及从37.8%（w/w）增加到48.2%（w/w），而碳水化合物从42.5%（w/w）降至26.2%（w/w）。用含2%二氧化碳的富氧空气培养的扁形栅藻平均生物量生产力最高，为91.25 mg L(-1) d(-1)，这相当于二氧化碳固定量为165 mg CO2 L(-1) d(-1)，脂质、蛋白质和碳水化合物的平均生产力分别为22.24、38.34和30.67 mg L(-1) d(-1)。
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