Liu Mingyan, Jiang Liqun, Yu Ze, Ma Meng, Chen Huiying, Pei Haiyan
School of Environmental Science and Engineering, Shandong University, Qingdao, 266237, China.
Department of Environmental Science and Engineering, Fudan University, Shanghai, 200433, China.
Bioresour Bioprocess. 2025 Jul 31;12(1):83. doi: 10.1186/s40643-025-00926-0.
Spirulina subsalsa represents a promising candidate for commercial production. Yet, due to its unique pattern of attached growth followed by upward flotation, there is an urgent need to find a suitable cultivation strategy and achieve scale-up. In the low-cost medium of seawater plus monosodium glutamate wastewater, this study initially investigated the influence of aeration rate (0, 0.3, 0.6 L/min) and reactor geometry (Dd, Dd, Dd D: diameter, cm; d: depth, cm) on microalgal growth, indicating that Spirulina subsalsa was suited for culturing in non-aerated, wide-shallow reactors. Then, in the single plastic reactor, diverse depths (2.25, 4.50, 9.00 cm) and surface areas (1000, 2000, and 3000 cm) for culturing Spirulina subsalsa were optimised to 4.5 cm and 2000 cm. Subsequently, a pilot-scale cultivation system with a volume of 162 L was constructed, adopting a semi-continuous culture mode. The average dry mass productivity was 0.12 g/L/d and 816.48 g of algal powder was harvested within 41 days. The mean contents of protein, carbohydrate and lipid were respectively 46.50%, 14.95% and 10.87%. Moreover, economic analysis demonstrated that the cost of produced algal powder was 8.30 USD/kg. In the future, Spirulina subsalsa has the potential to be developed into multiple products.
盐泽螺旋藻是商业生产的一个有前景的候选品种。然而，由于其独特的附着生长模式随后向上漂浮，迫切需要找到合适的培养策略并实现扩大规模。在海水加味精废水的低成本培养基中，本研究最初研究了曝气速率（0、0.3、0.6升/分钟）和反应器几何形状（Dd、Dd、Dd D：直径，厘米；d：深度，厘米）对微藻生长的影响，表明盐泽螺旋藻适合在非曝气的宽浅反应器中培养。然后，在单个塑料反应器中，将培养盐泽螺旋藻的不同深度（2.25、4.50、9.00厘米）和表面积（1000、2000和3000平方厘米）优化为4.5厘米和2000平方厘米。随后，构建了一个体积为162升的中试规模培养系统，采用半连续培养模式。平均干物质生产率为0.12克/升/天，在41天内收获了816.48克藻粉。蛋白质、碳水化合物和脂质的平均含量分别为46.50%、14.95%和10.87%。此外，经济分析表明，生产的藻粉成本为8.30美元/千克。未来，盐泽螺旋藻有潜力被开发成多种产品。
