Mnatsakanyan Hayk, Rico Patricia, Grigoriou Eleni, Candelas Aarón Maturana, Rodrigo-Navarro Aleixandre, Salmeron-Sanchez Manuel, Sabater i Serra Roser
†Center for Biomaterials and Tissue Engineering. Universitat Politècnica de València, 46022 València, Spain.
‡CIBER en Bioingeniería, Biomateriales y Nanomedicina, 46022 València, Spain.
ACS Appl Mater Interfaces. 2015 Aug 19;7(32):18125-35. doi: 10.1021/acsami.5b05466. Epub 2015 Aug 10.
Fibronectin fibrillogenesis is the physiological process by which cells elaborate a fibrous FN matrix. Poly(ethyl acrylate), PEA, has been described to induce a similar process upon simple adsorption of fibronectin (FN) from a protein solution-in the absence of cells-leading to the so-called material-driven fibronectin fibrillogenesis. Poly(methyl acrylate), PMA, is a polymer with very similar chemistry to PEA, on which FN is adsorbed, keeping the globular conformation of the protein in solution. We have used radical polymerization to synthesize copolymers with controlled EA/MA ratio, seeking to modulate the degree of FN fibrillogenesis. The physicochemical properties of the system were studied using dynamic-mechanical analysis, differential scanning calorimetry, and water contact angle. Both the degree of FN fibrillogenesis and the availability of the integrin binding region of FN directly depend on the percentage of EA in the copolymer, whereas the same total amount of FN was adsorbed regardless the EA/MA ratio. Cell morphology adhesion and differentiation of murine C2C12 were shown to depend on the degree of FN fibrillogenesis previously attained on the material surface. Myogenic differentiation was enhanced on the copolymers with higher EA content, i.e. more interconnected FN fibrils.
纤连蛋白纤维形成是细胞构建纤维状纤连蛋白基质的生理过程。聚（丙烯酸乙酯）（PEA）已被描述为在从蛋白质溶液中简单吸附纤连蛋白（FN）时（在无细胞的情况下）可诱导类似过程，从而导致所谓的材料驱动的纤连蛋白纤维形成。聚（丙烯酸甲酯）（PMA）是一种化学性质与PEA非常相似的聚合物，FN吸附在其上时，蛋白质在溶液中保持球状构象。我们使用自由基聚合来合成具有可控EA/MA比例的共聚物，试图调节FN纤维形成的程度。使用动态力学分析、差示扫描量热法和水接触角研究了该系统的物理化学性质。FN纤维形成的程度和FN整合素结合区域的可用性都直接取决于共聚物中EA的百分比，而无论EA/MA比例如何，吸附的FN总量相同。小鼠C2C12细胞的形态、黏附及分化被证明取决于先前在材料表面实现的FN纤维形成程度。在具有较高EA含量（即更多相互连接的FN纤维）的共聚物上，成肌分化得到增强。
