使用针对嗜吞噬细胞无形体柠檬酸合酶基因gltA的实时PCR检测法，检测挪威蓖麻硬蜱中的嗜吞噬细胞无形体。
Detection of Anaplasma phagocytophilum in Ixodes ricinus ticks from Norway using a realtime PCR assay targeting the Anaplasma citrate synthase gene gltA.
作者信息
Henningsson Anna J, Hvidsten Dag, Kristiansen Bjørn-Erik, Matussek Andreas, Stuen Snorre, Jenkins Andrew
机构信息
Department of Clinical Microbiology, Division of Medical Services, County Hospital Ryhov, Jönköping, Sweden.
Department of Microbiology and Infection Control, University Hospital of North Norway, Tromsø, Norway.
出版信息
BMC Microbiol. 2015 Aug 1;15:153. doi: 10.1186/s12866-015-0486-5.
BACKGROUND
A TaqMan real-time PCR assay targeting the Anaplasma citrate synthase gene, gltA, was developed and used for detection of Anaplasma phagocytophilum in 765 Ixodes ricinus ticks collected from dogs and cats in northern Norway (n = 669) and Telemark county in southern Norway (n = 96).
RESULTS
Among the ticks from northern Norway the prevalence of A. phagocytophilum was 3.0 %, while the prevalence in southern Norway was 2.1 % (p = 0.63). The gltA PCR assay showed a high analytical sensitivity (30 genomic units) and efficiency (98.5 %), and its utility in clinical diagnostics should be evaluated in future studies.
CONCLUSION
This is the first report of A. phagocytophilum occurrence in ticks collected north of the Arctic Circle in Norway. The prevalence is comparable to that found in Telemark county in southern Norway.
背景
开发了一种针对嗜吞噬细胞无形体柠檬酸合酶基因（gltA）的TaqMan实时PCR检测方法，并用于检测从挪威北部的狗和猫身上采集的765只蓖麻硬蜱（n = 669）以及挪威南部泰勒马克郡的96只蓖麻硬蜱中的嗜吞噬细胞无形体。
结果
在来自挪威北部的蜱中，嗜吞噬细胞无形体的患病率为3.0%，而在挪威南部为2.1%（p = 0.63）。gltA PCR检测方法显示出高分析灵敏度（30个基因组单位）和效率（98.5%），其在临床诊断中的实用性应在未来研究中进行评估。
结论
这是挪威北极圈以北采集的蜱中出现嗜吞噬细胞无形体的首次报告。患病率与挪威南部泰勒马克郡的相当。
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