Fusco Diana, Charbonneau Patrick
Program in Computational and Biology and Bioinformatics, Duke University, Durham, NC 27708, USA; Department of Chemistry, Duke University, Durham, NC 27708, USA; Department of Physics, University of California, Berkeley, CA 94720, USA; Department of Integrative Biology, University of California, Berkeley, CA 94720, USA.
Department of Chemistry, Duke University, Durham, NC 27708, USA; Department of Physics, Duke University, Durham, NC 27708, USA.
Colloids Surf B Biointerfaces. 2016 Jan 1;137:22-31. doi: 10.1016/j.colsurfb.2015.07.023. Epub 2015 Jul 14.
Crystallography may be the gold standard of protein structure determination, but obtaining the necessary high-quality crystals is also in some ways akin to prospecting for the precious metal. The tools and models developed in soft matter physics to understand colloidal assembly offer some insights into the problem of crystallizing proteins. This topical review describes the various analogies that have been made between proteins and colloids in that context. We highlight the explanatory power of patchy particle models, but also the challenges of providing guidance for crystallizing specific proteins. We conclude with a presentation of possible future research directions. This review is intended for soft matter scientists interested in protein crystallization as a self-assembly problem, and as an introduction to the pertinent physics literature for protein scientists more generally.
晶体学可能是蛋白质结构测定的金标准，但获得必要的高质量晶体在某些方面类似于勘探贵金属。软物质物理学中为理解胶体组装而开发的工具和模型为蛋白质结晶问题提供了一些见解。这篇专题综述描述了在这种背景下蛋白质与胶体之间所做的各种类比。我们强调了补丁粒子模型的解释力，但也指出了为特定蛋白质结晶提供指导所面临的挑战。我们最后介绍了可能的未来研究方向。这篇综述是为对蛋白质结晶作为一个自组装问题感兴趣的软物质科学家而写的，也是更广泛地为蛋白质科学家介绍相关物理文献的入门文章。
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