在皮秒时间尺度上研究视紫红质和异视紫红质的光化学。
Photochemistry of rhodopsin and isorhodopsin investigated on a picosecond time scale.
作者信息
Monger T G, Alfano R R, Callender R H
出版信息
Biophys J. 1979 Jul;27(1):105-15. doi: 10.1016/S0006-3495(79)85205-4.
Abstract
Bovine rhodopsin and isorhodopsin were excited with a single 530-nm, 7-ps light pulse emitted by a mode-locked Nd 3+ glass laser at room temperature. Within 3 ps of excitation, absorbance changes due to formation of bathorhodopsin were observed. The difference spectra generated during and 100 ps after pulse excitation are presented. The data show that bathorhodopsin formation is completed within 3 ps for both the primary pigments and suggest that a single common bathorhodopsin is photochemically formed from both primary pigments. Our findings provide additional support for the cis-trans isomerization model of the primary event in vision. Additional absorption transients that were observed near 670 and 460 nm are discussed.
摘要
在室温下，用锁模钕玻璃激光器发射的单个530纳米、7皮秒的光脉冲激发牛视紫红质和异视紫红质。在激发的3皮秒内，观察到由于视紫红质中间体形成而导致的吸光度变化。给出了脉冲激发期间和激发后100皮秒内产生的差示光谱。数据表明，两种主要色素的视紫红质中间体形成均在3皮秒内完成，这表明两种主要色素通过光化学作用形成了单一的共同视紫红质中间体。我们的发现为视觉初级事件的顺反异构化模型提供了额外的支持。还讨论了在670纳米和460纳米附近观察到的其他吸收瞬变现象。
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