Spalink J D, Reynolds A H, Rentzepis P M, Sperling W, Applebury M L
Proc Natl Acad Sci U S A. 1983 Apr;80(7):1887-91. doi: 10.1073/pnas.80.7.1887.
Bathorhodopsin-rhodopsin difference spectra of native 11-cis-rhodopsin and regenerated 9-cis-rhodopsin were measured at room temperature with a double-beam laser spectrophotometer after excitation at 532 nm. A detailed analysis of data obtained at 85 psec after excitation suggests that the bathorhodopsins generated from 11-cis- and 9-cis-rhodopsin differ in their extinction coefficients and that their absorption maxima are shifted in wavelength by about 10 nm from one another. The ratio of quantum yields for photochemical production of the 11-cis-bathorhodopsin and the 9-cis-bathorhodopsin approximates 1. Implications that the early photochemical processes in vision are more complex than previously considered are explored.
在室温下,用双光束激光分光光度计在532nm激发后,测量了天然11-顺式视紫红质和再生9-顺式视紫红质的视紫红质-视紫红质差光谱。对激发后85皮秒获得的数据进行的详细分析表明,由11-顺式和9-顺式视紫红质产生的视紫红质在消光系数上存在差异,并且它们的吸收最大值在波长上彼此偏移约10nm。11-顺式视紫红质和9-顺式视紫红质光化学产生的量子产率之比约为1。探讨了视觉早期光化学过程比先前认为的更为复杂的含义。