Tabrizi Leila, Mohammadi Siavash, Delshad Mojtaba, Moteshare Zadeh Babak
a Department of Horticultural Sciences , Faculty of Agricultural Sciences and Engineering, College of Agriculture and Natural Resources, University of Tehran , Karaj , Iran.
b Department of Soil Science , Faculty of Agricultural Engineering and Technology, College of Agriculture and Natural Resources, University of Tehran , Karaj , Iran.
Int J Phytoremediation. 2015;17(12):1244-52. doi: 10.1080/15226514.2015.1045131.
In order to study the effect of mycorrhizal fungi (inoculated and non-inoculated) and heavy metals stress [0, Pb (150 and 300 mg/kg) and Cd (40 and 80 mg/kg)] on pot marigold (Calendula officinalis L.), a factorial experiment was conducted based on a randomized complete block design with 4 replications in Research Greenhouse of Department of Horticultural Sciences, University of Tehran, Iran, during 2012-2013. Plant height, herbal and flower fresh and dry weight, root fresh and dry weight and root volume, colonization percentage, total petal extract, total petal flavonoids, root and shoot P and K uptakes, and Pb and Cd accumulations in root and shoot were measured. Results indicated that with increasing soil Pb and Cd concentration, growth and yield of pot marigold was reduced significantly; Cd had greater negative impacts than Pb. However, mycorrhizal fungi alleviated these impacts by improving plant growth and yield. Pot marigold concentrated high amounts of Pb and especially Cd in its roots and shoots; mycorrhizal plants had a greater accumulation of these metals, so that those under 80 mg/kg Cd soil(-1) accumulated 833.3 and 1585.8 mg Cd in their shoots and roots, respectively. In conclusion, mycorrhizal fungi can improve not only growth and yield of pot marigold in heavy metal stressed condition, but also phytoremediation performance by increasing heavy metals accumulation in the plant organs.
为了研究菌根真菌（接种和未接种）以及重金属胁迫[0、Pb（150和300毫克/千克）和Cd（40和80毫克/千克）]对金盏花（金盏菊）的影响，2012 - 2013年期间，在伊朗德黑兰大学园艺科学系的研究温室中，基于随机完全区组设计进行了一项析因试验，重复4次。测量了株高、地上部和花的鲜重与干重、根的鲜重与干重、根体积、定殖率、总花瓣提取物、总花瓣黄酮、根和地上部对P和K的吸收以及根和地上部中Pb和Cd的积累。结果表明，随着土壤中Pb和Cd浓度的增加，金盏花的生长和产量显著降低；Cd的负面影响大于Pb。然而，菌根真菌通过改善植物生长和产量减轻了这些影响。金盏花在其根和地上部积累了大量的Pb，尤其是Cd；菌根植物对这些金属的积累更多，例如在80毫克/千克Cd土壤（-1）条件下，菌根植物地上部和根中分别积累了833.3毫克和1585.8毫克Cd。总之，菌根真菌不仅可以改善重金属胁迫条件下金盏花的生长和产量，还可以通过增加植物器官中重金属的积累来提高植物修复性能。
