用于溶解蛋白质和药物制剂的低转变温度混合物（LTTMs）。
Low-Transition-Temperature Mixtures (LTTMs) for Dissolving Proteins and for Drug Formulation.
作者信息
Su Erzheng, Klibanov Alexander M
机构信息
Departments of Chemistry and Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
Enzyme and Fermentation Technology Laboratory, College of Light Industry Science and Engineering, Nanjing Forestry University, Nanjing, 210037, China.
出版信息
Appl Biochem Biotechnol. 2015 Oct;177(3):753-8. doi: 10.1007/s12010-015-1777-x. Epub 2015 Aug 4.
Several diverse proteins are found to readily dissolve in neat low-transition-temperature mixtures (LTTMs). They undergo no irreversible denaturation in such unusual solvents, and the resistance of hen egg-white lysozyme against thermoinactivation in LTTMs is greater than in aqueous solution at extreme pHs. Separately, the water-sensitive drug aspirin is found to form concentrated transparent LTTMs, where it is some 10-fold more stable against cleavage than in water.
人们发现几种不同的蛋白质很容易溶解在纯的低转变温度混合物（LTTMs）中。在这种不同寻常的溶剂中，它们不会发生不可逆的变性，并且在LTTMs中，鸡蛋清溶菌酶对热失活的抗性比在极端pH值的水溶液中更强。另外，人们发现对水敏感的药物阿司匹林能形成浓缩透明的LTTMs，在其中它比在水中对分解的稳定性大约高10倍。
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