Nakamura Akira
Faculty of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan.
Int J Syst Evol Microbiol. 2015 Nov;65(11):3878-3884. doi: 10.1099/ijsem.0.000508. Epub 2015 Aug 4.
Strain 43P was isolated as an l-glucose-utilizing bacterium from soil in Japan. Cells of the strain were Gram-stain-negative, aerobic and non-motile cocci. The 16S rRNA gene sequence of the strain showed high similarity to that of (98.5 %). Phylogenetic analyses based on 16S rRNA gene sequences revealed that this strain belongs to the genus Strain 43P contained Q-10 as the sole isoprenoid quinone. The major cellular fatty acids were Cω7 or Cω6 and C, and C, Cω9, C 3-OH and summed feature 2 were detected as minor components. The DNA G+C content of strain 43P was 64.1 mol%. Strain 43P contained the major polar lipids phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, an unknown aminolipid and two unknown glycolipids. The DNA-DNA relatedness between strain 43P and the six related type strains of the genus , including , was below 23 %. Based on the chemotaxonomic and physiological data and the values of DNA-DNA relatedness, especially the ability to assimilate l-glucose, this strain should be classified as a representative of a novel species of the genus , for which the name sp. nov. (type strain 43P = JCM 30587 = DSM 100094) is proposed.
菌株43P是从日本土壤中分离得到的一株利用L-葡萄糖的细菌。该菌株细胞为革兰氏阴性、需氧且不运动的球菌。该菌株的16S rRNA基因序列与[具体菌株名称未给出]的序列显示出高度相似性（98.5%）。基于16S rRNA基因序列的系统发育分析表明，该菌株属于[具体属名未给出]属。菌株43P含有Q-10作为唯一的类异戊二烯醌。主要的细胞脂肪酸为Cω7或Cω6以及C，C，Cω9，C 3-OH和总和特征2被检测为次要成分。菌株43P的DNA G+C含量为64.1mol%。菌株43P含有主要的极性脂质磷脂酰胆碱、磷脂酰甘油、二磷脂酰甘油、一种未知氨基脂质和两种未知糖脂。菌株43P与该属的六个相关模式菌株（包括[具体菌株名称未给出]）之间的DNA-DNA相关性低于23%。基于化学分类和生理数据以及DNA-DNA相关性值，特别是同化L-葡萄糖的能力，该菌株应被归类为[具体属名未给出]属一个新物种的代表，为此提出新物种名称[具体新物种名称未给出] sp. nov.（模式菌株43P = JCM 30587 = DSM 100094）。
