College of Biology and Environmental Sciences, Jishou University, Jishou, 416000, People's Republic of China.
Antonie Van Leeuwenhoek. 2011 May;99(4):753-60. doi: 10.1007/s10482-011-9549-2. Epub 2011 Jan 12.
A novel Gram-negative, catalase- and oxidase-positive, strictly aerobic, non spore-forming, rod-shaped bacterium, designated strain JSM 083058(T), was isolated from non-saline forest soil in Hunan Province, China. Growth occurred with 0-8% (w/v) NaCl (optimum, 0.5-3%) at pH 6.0-10.0 (optimum, pH 7.0) and at 5-35°C (optimum, 25-30°C). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain JSM 083058(T) fell within the cluster comprising species of the genus Sphingomonas, clustering with Sphingomonas aestuarii K4(T), with which it shared highest 16S rRNA gene sequence similarity (99.2%). The chemotaxonomic properties of strain JSM 083058(T) were consistent with those of the genus Sphingomonas. The predominant respiratory quinone was ubiquinone Q-10, and the major cellular fatty acids were summed feature 8 (C18:1ω7c/C18:1ω6c), C16:0, summed feature 3 (C16:1ω7c/C16:1ω6c) and C17:1ω6c. The polar lipids consisted of diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and sphingoglycolipid. The genomic DNA G+C content of strain JSM 083058(T) was 65.5 mol%. The combination of phylogenetic analysis, DNA-DNA relatedness, phenotypic characteristics and chemotaxonomic data supported the view that strain JSM 083058(T) represents a novel species of the genus Sphingomonas, for which the name Sphingomonas hunanensis sp. nov. is proposed. The type strain is JSM 083058(T) (=CCTCC AA 209011(T) = DSM 22213(T)).
一株新的革兰氏阴性、过氧化氢酶和氧化酶阳性、严格需氧、不产芽孢、杆状细菌，被命名为 JSM 083058(T) 菌株，从中国湖南省的非盐水森林土壤中分离得到。该菌在 0-8%（w/v）NaCl（最佳为 0.5-3%）、pH6.0-10.0（最佳为 pH7.0）和 5-35°C（最佳为 25-30°C）的条件下生长。基于 16S rRNA 基因序列的系统发育分析表明，JSM 083058(T) 菌株属于鞘氨醇单胞菌属的种，与鞘氨醇单胞菌属的海水 K4(T) 菌株聚为一簇，与该菌株的 16S rRNA 基因序列相似度最高（99.2%）。JSM 083058(T) 菌株的化学生物特征与鞘氨醇单胞菌属的特征一致。主要的呼吸醌是泛醌 Q-10，主要的细胞脂肪酸是 C18:1ω7c/C18:1ω6c、C16:0、C16:1ω7c/C16:1ω6c 和 C17:1ω6c。极性脂类由双磷脂酰甘油、磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰甘油和神经酰胺糖脂组成。菌株 JSM 083058(T) 的基因组 DNA G+C 含量为 65.5mol%。综合系统发育分析、DNA-DNA 同源性、表型特征和化学生物分类数据，支持 JSM 083058(T) 菌株代表鞘氨醇单胞菌属的一个新种，将其命名为湖南鞘氨醇单胞菌新种。模式菌株为 JSM 083058(T)（= CCTCC AA 209011(T) = DSM 22213(T)）。
