Yap C, Loh M T, Heng K K, Tan P, Yu S L, Chan S H, Ren E C
Department of Obstetrics and Gynaecology, Singapore General Hospital, Singapore.
Gynecol Obstet Invest. 2000;50(4):258-9. doi: 10.1159/000010327.
To determine if total nucleated cell counts alone are sufficient for predicting the efficacy of cord blood units for transplant from neonatal umbilical cord blood samples.
Umbilical cord blood samples were collected from 200 mothers at delivery and the cord blood units processed. The total nucleated cells and CD34+ cells were enumerated and compared for each sample.
Despite an apparent linear correlation between total nucleated cell counts and CD34+ cell counts, each group of total nucleated cell counts demonstrated a high degree of variation in CD34+ cell counts and could be as low as 0.1% of total nucleated cell counts.
Large variations in CD34+ cell counts per total nucleated cell count are present for cord blood units from neonatal umbilical cord samples. Hence a CD34+ cell count for each cord blood unit would improve selection of samples for transplant.
确定仅通过有核细胞总数是否足以从新生儿脐带血样本预测脐带血单位移植的疗效。
在分娩时从200名母亲处采集脐带血样本，并对脐带血单位进行处理。对每个样本的有核细胞总数和CD34+细胞进行计数和比较。
尽管有核细胞总数与CD34+细胞计数之间存在明显的线性相关性，但每组有核细胞总数的CD34+细胞计数都表现出高度的变异性，并且可能低至有核细胞总数的0.1%。
新生儿脐带样本的脐带血单位每有核细胞总数的CD34+细胞计数存在很大差异。因此，对每个脐带血单位进行CD34+细胞计数将改善移植样本的选择。
