Wahid Fadilah S Abdul, Nasaruddin Mona Zaria, Idris Mohd Razif Mohd, Tusimin Maiza, Tumian Nor Rafeah, Mahdy Zaleha Abdullah
Cell Therapy Centre, Department of Medicine, Universiti Kebangsaan Malaysia Medical Centre, Kuala Lumpur, Malaysia.
J Obstet Gynaecol Res. 2012 Mar;38(3):490-7. doi: 10.1111/j.1447-0756.2011.01740.x. Epub 2012 Feb 16.
To compare the numbers of cord blood CD34(+) hematopoietic stem cells (HSC) between preeclampsia (PE) and control (non-PE) subjects and to determine the factors that may influence this observation.
Umbilical cord blood was collected from 28 PE and 19 non-PE subjects. Nucleated and CD34(+) cell counts were derived using the Trucount tube-based stem cell enumeration kit on BD FACSCalibur.
The cord blood volume, nucleated and CD34(+) cell counts were significantly reduced in PE subjects compared to non-PE subjects. Among the PE subjects, systolic and diastolic blood pressure demonstrated a negative correlation with total nucleated and CD34(+) cell counts. Gestational age at delivery influenced cord blood volume and nucleated cell counts, but not CD34(+) cell counts. Birth weight and placental weight correlated strongly with cord blood volume, and nucleated and CD34(+) cell counts. There were no correlations observed between cord blood parameters and maternal age, maternal white cell count, gravidity, route of delivery or neonatal gender among PE subjects.
Preeclampsia has a negative impact on the yield of HSC obtained from cord blood at delivery. Maternal blood pressure, neonatal birth weight and placental weight are important factors influencing the numbers of cord blood HSC. These findings should be taken into consideration when selecting cord blood units from mothers with PE for banking. Selecting the heaviest term neonate might improve the yield of cord blood HSC obtained from PE mothers.
比较子痫前期（PE）患者与对照组（非PE）脐血中CD34(+)造血干细胞（HSC）的数量，并确定可能影响该观察结果的因素。
收集28例PE患者和19例非PE患者的脐血。使用基于Trucount管的干细胞计数试剂盒在BD FACSCalibur上进行有核细胞和CD34(+)细胞计数。
与非PE患者相比，PE患者的脐血体积、有核细胞和CD34(+)细胞计数显著降低。在PE患者中，收缩压和舒张压与总核细胞和CD34(+)细胞计数呈负相关。分娩时的孕周影响脐血体积和有核细胞计数，但不影响CD34(+)细胞计数。出生体重和胎盘重量与脐血体积、有核细胞和CD34(+)细胞计数密切相关。在PE患者中，未观察到脐血参数与母亲年龄、母亲白细胞计数、妊娠次数、分娩方式或新生儿性别之间的相关性。
子痫前期对分娩时从脐血中获取的造血干细胞产量有负面影响。母亲血压、新生儿出生体重和胎盘重量是影响脐血造血干细胞数量的重要因素。从患有PE的母亲中选择脐血单位进行储存时应考虑这些发现。选择体重最重的足月儿可能会提高从PE母亲那里获得的脐血造血干细胞产量。
