采用响应面法优化超声波辅助从番石榴（Psidium guajava L.）叶中提取抗氧化化合物的工艺
Optimization of ultrasonic-assisted extraction of antioxidant compounds from Guava (Psidium guajava L.) leaves using response surface methodology.
作者信息
Kong Fansheng, Yu Shujuan, Feng Zeng, Wu Xinlan
机构信息
College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, PR China ; Department of Pharmaceutical Engineering, College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510640, PR China ; Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Guangzhou 510640, PR China.
College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, PR China ; Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Guangzhou 510640, PR China.
出版信息
Pharmacogn Mag. 2015 Jul-Sep;11(43):463-9. doi: 10.4103/0973-1296.160451.
OBJECTIVE
To optimization of extraction of antioxidant compounds from guava (Psidium guajava L.) leaves and showed that the guava leaves are the potential source of antioxidant compounds.
MATERIALS AND METHODS
The bioactive polysaccharide compounds of guava leaves (P. guajava L.) were obtained using ultrasonic-assisted extraction. Extraction was carried out according to Box-Behnken central composite design, and independent variables were temperature (20-60°C), time (20-40 min) and power (200-350 W). The extraction process was optimized by using response surface methodology for the highest crude extraction yield of bioactive polysaccharide compounds.
RESULTS
The optimal conditions were identified as 55°C, 30 min, and 240 W. 1,1-diphenyl-2-picryl-hydrazyl and hydroxyl free radical scavenging were conducted.
CONCLUSION
The results of quantification showed that the guava leaves are the potential source of antioxidant compounds.
目的
优化从番石榴（Psidium guajava L.）叶中提取抗氧化化合物的方法，并表明番石榴叶是抗氧化化合物的潜在来源。
材料与方法
采用超声辅助提取法获得番石榴（Psidium guajava L.）叶中的生物活性多糖化合物。提取按照Box-Behnken中心复合设计进行，自变量为温度（20 - 60°C）、时间（20 - 40分钟）和功率（200 - 350瓦）。采用响应面法对生物活性多糖化合物的最高粗提产率进行提取工艺优化。
结果
确定最佳条件为55°C、30分钟和240瓦。进行了1,1 - 二苯基 - 2 - 苦基肼自由基和羟基自由基清除实验。
结论
定量结果表明番石榴叶是抗氧化化合物的潜在来源。
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