Li Hui, Chen Bo, Yao Shouzhuo
State Key Laboratory of Chemo/Biological Sensing and Chemometrics, Hunan University, Changsha 410082, PR China.
Ultrason Sonochem. 2005 Mar;12(4):295-300. doi: 10.1016/j.ultsonch.2004.01.033.
An ultrasonic method for the extraction of chlorogenic acid from fresh leaves of Eucommia ulmodies Oliv. was investigated and optimized. The influence of four extraction variables on extraction efficiency of chlorogenic acid was investigated. The optimum extraction conditions found were: 70% aqueous methanol; solvent: sample ratio=20:1 (v/w); extraction time 3 x 30 min. The recovery of chlorogenic acid was studied (HPLC) and the reproducibility of the extraction method was determined. The optimized ultrasonic extraction conditions were applied to extract chlorogenic acid from fresh leaves, fresh bark and dried bark of E. ulmodies and four traditional Chinese medicines. The application of sonication method was shown to be highly efficient in the extraction of chlorogenic acid from E. ulmodies and other Chinese medicines compared with classical methods.
研究并优化了一种从杜仲鲜叶中提取绿原酸的超声方法。考察了四个提取变量对绿原酸提取效率的影响。发现的最佳提取条件为：70%甲醇水溶液；溶剂与样品比例=20:1（v/w）；提取时间3×30分钟。研究了绿原酸的回收率（高效液相色谱法）并测定了提取方法的重现性。将优化后的超声提取条件应用于从杜仲的鲜叶、鲜皮和干皮以及四种中药中提取绿原酸。结果表明，与传统方法相比，超声处理方法在从杜仲和其他中药中提取绿原酸方面具有很高的效率。
