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显微镜技术和高效液相色谱法在款冬花（款冬）质量评估中的应用

Application of microscopy technique and high-performance liquid chromatography for quality assessment of the flower bud of Tussilago farfara L. (Kuandonghua).

作者信息

Li Da, Liang Li, Zhang Jing, Kang Tingguo

机构信息

School of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian, China.

School of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian, China ; School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China.

出版信息

Pharmacogn Mag. 2015 Jul-Sep;11(43):594-600. doi: 10.4103/0973-1296.160473.

DOI:10.4103/0973-1296.160473
PMID:26246737
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4522848/
Abstract

BACKGROUND

Quality control is one of the bottleneck problems limiting the application and development of traditional Chinese medicine (TCM). In recent years, microscopy and high-performance liquid chromatography (HPLC) techniques have been frequently applied in the quality control of TCM. However, studies combining conventional microscopy and HPLC techniques for the quality control of the flower bud of Tussilago farfara L. (Kuandonghua) have not been reported.

OBJECTIVE

This study was undertaken to evaluate the quality of the flower bud of T. farfara L. and to establish the relationships between the quantity of pollen grains and four main bioactive constituents: tussilagone, chlorogenic acid, rutin and isoquercitrin.

MATERIALS AND METHODS

In this study, microscopic examination was used to quantify microscopic characteristics of the flower bud of T. farfara L., and the chemical components were determined by HPLC. The data were analyzed by Statistical Package for the Social Sciences statistics software.

RESULTS

The results of the analysis showed that tussilagone, chlorogenic acid, rutin and isoquercitrin were significantly correlated with the quantity of pollen grains in the flower bud of T. farfara L. There is a positive correlation between them. From these results, it can be deduced that the flower bud of T. farfara L. with a greater quantity of pollen grains should be of better quality.

CONCLUSION

The study showed that the established method can be helpful for evaluating the quality of the flower bud of T. farfara L. based on microscopic characteristic constants and chemical quantitation.

摘要

背景

质量控制是限制中药应用与发展的瓶颈问题之一。近年来，显微镜技术和高效液相色谱（HPLC）技术在中药质量控制中被频繁应用。然而，尚未见将传统显微镜技术与HPLC技术结合用于款冬花（Kuandonghua）花蕾质量控制的研究报道。

目的

本研究旨在评价款冬花花蕾的质量，并建立花粉粒数量与4种主要生物活性成分（款冬酮、绿原酸、芦丁和异槲皮苷）之间的关系。

材料与方法

本研究采用显微镜检查法定量款冬花花蕾的微观特征，并用HPLC法测定其化学成分。数据采用社会科学统计软件包进行分析。

结果

分析结果显示，款冬酮、绿原酸、芦丁和异槲皮苷与款冬花花蕾中的花粉粒数量显著相关，它们之间呈正相关。由此结果可推断，花粉粒数量较多的款冬花花蕾质量更佳。

结论

本研究表明，所建立的方法有助于基于微观特征常数和化学定量评价款冬花花蕾的质量。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/192c/4522848/d3097bc95fa5/PM-11-594-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/192c/4522848/0b923f1d4088/PM-11-594-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/192c/4522848/8d80af76a2f4/PM-11-594-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/192c/4522848/d3097bc95fa5/PM-11-594-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/192c/4522848/0b923f1d4088/PM-11-594-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/192c/4522848/8d80af76a2f4/PM-11-594-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/192c/4522848/d3097bc95fa5/PM-11-594-g006.jpg

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