Seo U Min, Zhao Bing Tian, Kim Won Il, Seo Eun Kyoung, Lee Jae Hyun, Min Byung Sun, Shin Beom Soo, Son Jong Keun, Woo Mi Hee
College of Pharmacy, Catholic University of Daegu.
Chem Pharm Bull (Tokyo). 2015;63(7):546-53. doi: 10.1248/cpb.c15-00204. Epub 2015 May 14.
The flower bud of Tussilago farfara L., called Farfarae Flos, has traditionally been used in Oriental medicine for the treatment of bronchitis and asthma. To establish a standard for quality control as well as the reliable identification of Farfarae Flos, the contents of five standards, rutin (1), isoquercetin (2), 3,5-dicaffeoylquinic acid (3), tussilagone (4), and tussilagonone (5), were determined by quantitative high-performance liquid chromatography (HPLC)/photodiode array (PDA) analysis. The five standards were separated on a YoungJinBioChrom Aegispak C18-L (250-mm×4.6-mm, 5-µm) column by gradient elution using 0.03% trifluoroacetic acid in water (A), with acetonitrile (B) as the mobile phase. The flow rate was 1.0 mL/min, and the UV detector wavelength was set at 220 nm. The method was successfully used in the analysis of Farfarae Flos from different geographic origins with relatively simple conditions and procedures, and the results demonstrated satisfactory linearity, recovery, precision, accuracy, stability, and robustness. The HPLC analytical method for pattern recognition analysis was validated by repeated analysis of 62 Farfarae Flos samples. This result indicated that the established HPLC/PDA method is suitable for quantitation and pattern recognition analyses for the quality evaluation of Farfarae Flos.
款冬花，又称款冬花，其花蕾在传统东方医学中用于治疗支气管炎和哮喘。为了建立质量控制标准以及可靠鉴定款冬花，采用定量高效液相色谱（HPLC）/光电二极管阵列（PDA）分析法测定了五种标准品芦丁（1）、异槲皮苷（2）、3,5-二咖啡酰奎宁酸（3）、款冬酮（4）和款冬新酮（5）的含量。五种标准品在YoungJinBioChrom Aegispak C18-L（250mm×4.6mm，5μm）柱上分离，以0.03%三氟乙酸水溶液（A）和乙腈（B）为流动相进行梯度洗脱。流速为1.0 mL/min，紫外检测器波长设定为220nm。该方法以相对简单的条件和步骤成功用于分析不同地理来源的款冬花，结果显示出良好的线性、回收率、精密度、准确度、稳定性和耐用性。通过对62个款冬花样品的重复分析，验证了用于模式识别分析的HPLC分析方法。该结果表明，所建立的HPLC/PDA方法适用于款冬花质量评价的定量和模式识别分析。
