Wu Di, Wang Zhengtao, Zhang Mian
Department of Pharmacognosy, China Pharmaceutical University, Nanjing 211198, China.
Zhongguo Zhong Yao Za Zhi. 2010 Oct;35(20):2722-5.
To develop an HPLC method for simultaneous determination of rutin, isoquercitrin and chlorogenic acid in Farfarae Flos.
The analysis was carried out on a Phenomenex Synergi POLAR-RP 80A column (4.6 mm x 250 mm, 4 microm) with gradient elution using methanol-acetonitrile-water (adjusted to pH 2.5 with formic acid) as mobile phase. The flow rate was 1.2 mL x min(-1) and the detection wavelength was at 255 nm.
The calibration curves were linear over the range of 0.2-2 000 microg x L(-1) for rutin and isoquercitrin, 10-2 000 microg x L(-1) for chlorogenic acid, respectively. The average recoveries were 99.5% for rutin, 100.1% for isoquercitrin and 99.4% for chlorogenic acid, respectively, with RSD not more than 3.0%.
The described method is reliable and could be used for the quality control of Farfarae Flos.
建立同时测定款冬花中芦丁、异槲皮苷和绿原酸的高效液相色谱法。
采用Phenomenex Synergi POLAR-RP 80A色谱柱（4.6 mm×250 mm，4 µm），以甲醇-乙腈-水（用甲酸调至pH 2.5）为流动相进行梯度洗脱。流速为1.2 mL·min⁻¹，检测波长为255 nm。
芦丁和异槲皮苷在0.2 - 2 000 μg·L⁻¹范围内、绿原酸在10 - 2 000 μg·L⁻¹范围内线性关系良好。芦丁、异槲皮苷和绿原酸的平均回收率分别为99.5%、100.1%和99.4%，相对标准偏差均不超过3.0%。
该方法可靠，可用于款冬花的质量控制。
