Tease L A, Fazleabas A T, Chan W Y
Laboratory of Protein Studies, Oklahoma Medical Research Foundation, Oklahoma City 73104.
Biol Reprod. 1989 Dec;41(6):1113-21. doi: 10.1095/biolreprod41.6.1113.
Immunostaining of baboon placental tissues with anti-human pregnancy-specific beta 1-glycoprotein (SPI) antibodies demonstrated that an SP1-like molecule was present in the syncytiotrophoblasts. Staining was observed on the membrane and in the cytoplasm, but the nucleus was devoid of any staining. Western blot analysis further demonstrated the presence of five protein species in baboon placental extract, whereas four protein bands were detected in human placental extract. Culture medium of baboon placental villi also contained five SP1-like molecules with sizes slightly different from those present in the placental extract. Amniotic fluid and culture medium of decidua basalis and chorioamniotic tissue contained lesser quantities and fewer species of SP1-like molecules. However, an 87 kDa band was present in all samples examined. Northern blot analysis of baboon placenta with a human placental SP1 cDNA probe demonstrated the presence of a 1.65 Kb band, whereas two hybridizing bands (1.65 Kb and 2.25 Kb) were present in human placenta. Southern blot analysis of baboon genomic DNA further demonstrated the presence of multiple bands hybridizing with a human placental SP1 cDNA probe. These results showed the presence in baboons of multiple genes encoding mRNAs and proteins highly similar to human placental SP1.
用抗人妊娠特异性β1-糖蛋白（SPI）抗体对狒狒胎盘组织进行免疫染色显示，合体滋养层细胞中存在一种类似SP1的分子。在细胞膜和细胞质中观察到染色，但细胞核无任何染色。蛋白质印迹分析进一步证明狒狒胎盘提取物中存在五种蛋白质，而在人胎盘提取物中检测到四条蛋白带。狒狒胎盘绒毛的培养基中也含有五种类似SP1的分子，其大小与胎盘提取物中的分子略有不同。基底蜕膜和绒毛羊膜组织的羊水和培养基中类似SP1的分子数量较少且种类较少。然而，在所检测的所有样本中均存在一条87 kDa的条带。用人胎盘SP1 cDNA探针进行的狒狒胎盘Northern印迹分析显示存在一条1.65 Kb的条带，而人胎盘中存在两条杂交带（1.65 Kb和2.25 Kb）。狒狒基因组DNA的Southern印迹分析进一步证明存在多条与人胎盘SP1 cDNA探针杂交的条带。这些结果表明，狒狒中存在多个编码与人类胎盘SP1高度相似的mRNA和蛋白质的基因。
