Shupert W L, Chan W Y
Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center.
Mol Cell Biochem. 1993 Mar 24;120(2):159-70. doi: 10.1007/BF00926089.
Pregnancy-specific beta 1-glycoprotein (PSG) transcripts have been identified in a number of placental and non-placental tissues. Using a placental PSG cDNA probe to screen a normal human intestinal cDNA library we have isolated 22 hybridizing clones. These clones could be divided into four groups. Nucleotide sequence analysis showed that one group of clones correspond to functional and another group correspond to non-functional PSG cDNAs. The other two groups are homologous to the nonspecific cross-reacting antigen (NCA) and biliary glycoprotein (BGP), both of which are members of the carcinoembryonic antigen (CEA) family. Thus, PSG, NCA and BGP are co-expressed in normal human intestine. RNA and immunoblot analysis, along with polymerase chain reaction amplification further confirm the expression of PSG in human intestinal tissue.
妊娠特异性β1-糖蛋白（PSG）转录本已在多种胎盘组织和非胎盘组织中被鉴定出来。利用胎盘PSG cDNA探针筛选正常人肠道cDNA文库，我们分离出了22个杂交克隆。这些克隆可分为四组。核苷酸序列分析表明，一组克隆对应于功能性PSG cDNA，另一组对应于非功能性PSG cDNA。另外两组与非特异性交叉反应抗原（NCA）和胆汁糖蛋白（BGP）同源，这两者都是癌胚抗原（CEA）家族的成员。因此，PSG、NCA和BGP在正常人肠道中共同表达。RNA和免疫印迹分析以及聚合酶链反应扩增进一步证实了PSG在人肠道组织中的表达。
