Wang G H
Zhonghua Liu Xing Bing Xue Za Zhi. 1989 Dec;10(6):363-5.
One thousand and two hundred samples of donor blood, negative for HBsAg by RPHA, were retested by ELISA and 29 samples were found to be positive for HBsAg. Of 29 HBsAg positive donors, all were negative for IgM-anti HBc, 11 were positive for HBeAg (37.93%), 6 were positive for anti-HBe (20.69%), 10 were positive for HBVDNA (34.48%). Of 29 HBsAg positive samples, the high titers (greater than or equal to 1:500) samples accounted for 17.25 per cent of the total. It was suggested that RPHA should be replaced by ELISA or RIA in screening blood donors for HBsAg and quality control of laboratories for screening blood donors should be made by increasing technical competence of technicians.
1200份经反向被动血凝试验（RPHA）检测乙肝表面抗原（HBsAg）呈阴性的供血者血液样本，采用酶联免疫吸附测定（ELISA）进行复检，发现29份样本HBsAg呈阳性。在这29名HBsAg阳性供血者中，所有样本IgM抗乙肝核心抗体（IgM-anti HBc）均为阴性，11份样本乙肝e抗原（HBeAg）呈阳性（37.93%），6份样本乙肝e抗体（anti-HBe）呈阳性（20.69%），10份样本乙肝病毒脱氧核糖核酸（HBVDNA）呈阳性（34.48%）。在29份HBsAg阳性样本中，高滴度（大于或等于1:500）样本占总数的17.25%。建议在供血者HBsAg筛查中，用ELISA或放射免疫分析（RIA）取代RPHA，并通过提高技术人员的技术能力对供血者筛查实验室进行质量控制。
