Department of Anatomy and Cell Biology, University of Saskatchewan, S7N5E5, Canada; Division of Biomedical Engineering, University of Saskatchewan, S7N5A9, Canada.
Department of Mechanical Engineering, University of Saskatchewan, S7N5A9, Canada; Division of Biomedical Engineering, University of Saskatchewan, S7N5A9, Canada.
Mater Sci Eng C Mater Biol Appl. 2015 Nov 1;56:251-9. doi: 10.1016/j.msec.2015.06.015. Epub 2015 Jun 11.
Poly(lactic-co-glycolic acid) (PLGA) microspheres and PLGA/alginate composite microspheres were prepared by a novel double emulsion and solvent evaporation technique and loaded with bovine serum albumin (BSA) or rabbit anti-laminin antibody protein. The addition of alginate and the use of a surfactant during microsphere preparation increased the encapsulation efficiency and reduced the initial burst release of hydrophilic BSA. Confocal laser scanning microcopy (CLSM) of BSA-loaded PLGA/alginate composite microspheres showed that PLGA, alginate, and BSA were distributed throughout the depths of microspheres; no core/shell structure was observed. Scanning electron microscopy revealed that PLGA microspheres erode and degrade more quickly than PLGA/alginate composite microspheres. When loaded with anti-laminin antibody, the function of released antibody was well preserved in both PLGA and PLGA/alginate composite microspheres. The biocompatibility of PLGA and PLGA/alginate microspheres were examined using four types of cultured cell lines, representing different tissue types. Cell survival was variably affected by the inclusion of alginate in composite microspheres, possibly due to the sensitivity of different cell types to excess calcium that may be released from the calcium cross-linked alginate.
聚（乳酸-共-乙醇酸）（PLGA）微球和 PLGA/藻酸盐复合微球通过一种新的双重乳液和溶剂蒸发技术制备，并负载牛血清白蛋白（BSA）或兔抗层粘连蛋白抗体蛋白。藻酸盐的添加和在微球制备过程中使用表面活性剂提高了包封效率并减少了亲水性 BSA 的初始突释。载有 BSA 的 PLGA/藻酸盐复合微球的共焦激光扫描显微镜（CLSM）显示，PLGA、藻酸盐和 BSA 分布在微球的整个深度；没有观察到核/壳结构。扫描电子显微镜显示，PLGA 微球比 PLGA/藻酸盐复合微球更快地侵蚀和降解。当负载抗层粘连蛋白抗体时，释放的抗体的功能在 PLGA 和 PLGA/藻酸盐复合微球中都得到了很好的保留。使用四种不同组织类型的培养细胞系，研究了 PLGA 和 PLGA/藻酸盐微球的生物相容性。藻酸盐的存在对复合微球中细胞的存活有不同的影响，这可能是由于不同细胞类型对可能从钙交联藻酸盐中释放的过量钙的敏感性不同。
