Slominskiĭ P A, Maleeva N E, Buiakova O I, Tsar'kov S G, Panina T N, Ryskov A P, Limborskaia S A
Genetika. 1989 Nov;25(11):1925-36.
Construction of a human cortex cDNA bank is described as well as the isolation from this bank of pBH71 and pBH3 clones with preferential expression in nervous and in tumor cells. The clones can be included into the third class of cDNA according to Sutcliff's classification. The mRNA corresponding to this cDNA class is considered to play the key role in determination of specificity of nervous tissue. Expression of the pBH71 sequence was revealed in human cortex and in tissues of different genesis (from neuroblastoma to uterus myoma), a 2 kb mRNA which corresponds to one and the same cDNA chain having been found in all tissues under analysis. The nucleotide sequence of cDNA insertion into the pBH71 clone of 447 n.p. was determined, and particular features of cDNA nucleotide composition and possible schemes of its translation were analysed. Weak homology was found between the 3'-end of cDNA insertion of the pBH71 clone and the 3'-end region of human proopiomelanocortine. The cDNA of the pBH3 clone hybridizes with the 0.8 kb mRNA revealed in human cortex and neuroendocrine tumors of different nature. No homology was revealed between the cDNA sequence of the pBH3 clone and any genes deciphered.
本文描述了人皮质cDNA文库的构建，以及从该文库中分离出在神经细胞和肿瘤细胞中优先表达的pBH71和pBH3克隆。根据萨克利夫的分类，这些克隆可归入第三类cDNA。对应于这类cDNA的mRNA被认为在神经组织特异性的决定中起关键作用。在人皮质和不同起源的组织（从神经母细胞瘤到子宫肌瘤）中发现了pBH71序列的表达，在所有分析的组织中都发现了一条2kb的mRNA，它对应于同一条cDNA链。测定了插入pBH71克隆的447个核苷酸的cDNA的核苷酸序列，并分析了cDNA核苷酸组成的特点及其可能的翻译方案。发现pBH71克隆的cDNA插入片段的3'末端与人促阿片黑素细胞皮质素的3'末端区域有弱同源性。pBH3克隆的cDNA与在人皮质和不同性质的神经内分泌肿瘤中发现的0.8kb mRNA杂交。未发现pBH3克隆的cDNA序列与任何已破译的基因有同源性。
