Du C A, Conklin J L
Digestive Diseases Core Center, University of Iowa College of Medicine, Iowa City 52242.
J Auton Nerv Syst. 1989 Nov;28(2):167-77. doi: 10.1016/0165-1838(89)90089-1.
Slow oscillations of the membrane potential difference of circular smooth muscle cells of the colon, slow waves, arise at the interface between the submucosa and muscularis externa of the colon. These studies test the hypothesis that the generation of slow waves in the circular muscle layer of the colon of the cat is dependent upon an intact interface between the submucosa and muscularis externa. Electrical recordings were made from 3 types of tissue preparations from the proximal colon: (a) submucosa and muscularis externa intact, (b) submucosa isolated from the circular muscle, and (c) muscularis externa isolated from the submucosa. The cellular make-up of each tissue was determined by the osmic acid-zinc iodide method (Champy-Maillet), and the Masson's trichrome stain. Continuous slow waves were recorded from intact tissues. Removal of the submucosa abolished slow waves from the underlying muscularis externa. Either irregular electrical transients or waxing and waning slow wave-like activity were recorded from the isolated submucosa. Submucosal tissues removed from the muscularis externa contained no circular smooth muscle, but did contain elements of the plexus submucosus extremus.
结肠环形平滑肌细胞的膜电位差缓慢振荡，即慢波，出现在结肠黏膜下层和外肌层之间的界面处。这些研究检验了这样一个假说：猫结肠环形肌层中慢波的产生依赖于黏膜下层和外肌层之间完整的界面。从近端结肠的3种组织制备物中进行电记录：(a) 黏膜下层和外肌层完整；(b) 黏膜下层与环形肌分离；(c) 外肌层与黏膜下层分离。每种组织的细胞组成通过锇酸 - 碘化锌法（尚皮 - 马耶法）和马森三色染色法确定。从完整组织中记录到连续的慢波。去除黏膜下层会消除其下方外肌层的慢波。从分离的黏膜下层记录到不规则的电瞬变或起伏的慢波样活动。从外肌层移除的黏膜下组织不含环形平滑肌，但确实含有黏膜下终末丛的成分。
