Conklin J L, Du C
Center for Digestive Diseases, University of Iowa Hospitals and Clinics, Iowa City 52242.
Am J Physiol. 1990 Jun;258(6 Pt 1):G894-903. doi: 10.1152/ajpgi.1990.258.6.G894.
Colonic slow waves (SWs) are generated by nonneuronal cells located at the interface of the submucosa and muscularis propria. It has been proposed that SWs arise from a complex of nerves, interstitial cells of Cajal, and smooth muscle found at this location. These experiments test the hypothesis that the propagation of colonic SWs depends on an intact interface between the submucosa and muscularis propria. The electromyogram was recorded from segments of the proximal colon of the cat. All intact tissues generated SWs that propagated in the long and circumferential axes of the colon. Tetrodotoxin did not disrupt SW propagation in either axis. Transection of tissues between recording sites interrupted the spread of SWs in both axes. Transection of the submucosa disrupted the longitudinal spread of SWs, whereas transection of the muscularis propria did not. Removing the submucosa from the midportion of tissue segments oriented in the long axis of the colon resulted in a loss of SWs from the segment devoid of submucosa. Transection of the submucosa of tissue segments oriented in the circular axis of the colon did not disrupt circumferential propagation of SWs. Dissecting a 1-cm-wide segment of submucosa from the midportion of such a circularly oriented tissue did not disrupt the circumferential spread of SWs, and SWs were recorded from the muscle segment that was devoid of submucosa. SWs were not recorded from the segment devoid of submucosa when it was isolated from adjacent intact segments. The data support the hypothesis that the regeneration of SWs during their longitudinal propagation takes place at the interface between the submucosa and muscularis propria.
结肠慢波（SWs）由位于黏膜下层和固有肌层交界处的非神经细胞产生。有人提出，慢波源于该位置发现的神经、Cajal间质细胞和平滑肌的复合体。这些实验检验了结肠慢波传播依赖于黏膜下层和固有肌层之间完整界面这一假说。从猫近端结肠段记录肌电图。所有完整组织均产生在结肠长轴和圆周轴上传播的慢波。河豚毒素并未破坏任一轴向上的慢波传播。记录部位之间的组织横断会中断两个轴向上慢波的传播。黏膜下层横断会破坏慢波的纵向传播，而固有肌层横断则不会。从沿结肠长轴定向的组织段中部去除黏膜下层会导致无黏膜下层的节段失去慢波。沿结肠圆周轴定向的组织段黏膜下层横断不会破坏慢波的圆周传播。从这种圆周定向组织的中部剥离1厘米宽的黏膜下层节段不会破坏慢波的圆周传播，并且从无黏膜下层的肌段记录到了慢波。当无黏膜下层的节段与相邻完整节段分离时，未记录到慢波。这些数据支持了慢波在纵向传播过程中的再生发生在黏膜下层和固有肌层之间界面这一假说。
