Pournourali M, Tarang A R, Yousefi M
Faculty of Sciences, University of Guilan Department of Biology Rasht Iran mostafapournourali@yahoo.com.
Agriculture Biotechnology Research Institute of Iran (ABRII) Department of Genomics and Animal, Branches of north region of Iran Rasht Iran.
Cell Mol Biol (Noisy-le-grand). 2015 Aug 5;61(4):21-4.
Prostate cancer (PCa) is the most common malignancy in men and the fourth most common cause of death based on cancer all over the world. Many genes has been shown to be involved in the progress of the prostate cancer. Human apurinic/apyrimidinic endonuclease 1 (APE1) is a multifunctional protein that has an important role in the base excision repair (BER) pathway. The aim of this study was to evaluate the association of ApE1 1349T>G polymorphism and the susceptibility to prostate cancer in northern Iran population. Samples were collected from 100 patients diagnosed with prostate cancer patients and 100 controls subjects and genotyped by PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism). We observed a significant difference in genotype distributions of ApE1 1349T>G polymorphism between patients and controls (P= 0.039). Our findings revealed individuals with the variant TG and GG had a significant increased risk of prostate cancer (GG: OR= 2.50, 95%CI= 1.063-5.874, P= 0.035. TG: OR= 2.40, 95%CI= 1.16-4.95, P= 0.017). Also, more analyses were showed that G allele were associated with increased risk of prostate cancer (OR= 1.493, 95%CI= 1.007-2.21, P= 0.045). The data from this study indicates that the ApE1 1349T>G polymorphism is associated with increased risk of prostate cancer. Although more studies should be considered with larger number of patients and control subjects to confirm our results.
前列腺癌（PCa）是男性中最常见的恶性肿瘤，也是全球癌症相关死亡的第四大常见原因。许多基因已被证明与前列腺癌的进展有关。人脱嘌呤/脱嘧啶内切酶1（APE1）是一种多功能蛋白质，在碱基切除修复（BER）途径中起重要作用。本研究的目的是评估伊朗北部人群中ApE1 1349T>G多态性与前列腺癌易感性的关联。从100例确诊为前列腺癌的患者和100例对照受试者中采集样本，并通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）进行基因分型。我们观察到患者和对照之间ApE1 1349T>G多态性的基因型分布存在显著差异（P = 0.039）。我们的研究结果显示，携带变异型TG和GG的个体患前列腺癌的风险显著增加（GG：比值比（OR）= 2.50，95%置信区间（CI）= 1.063 - 5.874，P = 0.035；TG：OR = 2.40，95%CI = 1.16 - 4.95，P = 0.017）。此外，更多分析表明，G等位基因与前列腺癌风险增加相关（OR = 1.493，95%CI = 1.007 - 2.21，P = 0.045）。本研究的数据表明，ApE1 1349T>G多态性与前列腺癌风险增加有关。尽管应该通过更多患者和对照受试者进行更多研究以证实我们的结果。
