Mashayekhi F, Yousefi M, Salehi Z, Saedi H S, Pournourali M
University of Guilan Department of Biology, Faculty of Sciences Rasht Iran mashayekhi@guilan.ac.ir.
University of Guilan Department of Biology, Faculty of Sciences Rasht Iran.
Cell Mol Biol (Noisy-le-grand). 2015 Aug 28;61(4):70-4.
Breast cancer is the most common cancer and one of the main causes of cancer-related death in the world and has become a major public health concern. Many genes have been shown to be involved in the progress of the breast cancer. Human apurinic/apyrimidinic endonuclease 1 (APE1) is a multifunctional protein that has an important role in the base excision repair (BER) pathway. The aim of this study was to evaluate the association between ApE1 -656T>G and 1349T>G polymorphisms and the susceptibility to breast cancer in northern Iran population. Samples were obtained from 150 patients diagnosed with breast cancer and 150 control subjects and genotyped by tetra - primer amplification refractory mutation system PCR (T-ARMS-PCR). We observed a significant difference in genotype distributions of -656T>G polymorphism between patients and controls (P= 0.03). Findings indicated that individuals with the variant TG genotypes had a significant decreased risk of breast cancer (OR= 0.55, 95% CI= 0.33 - 0.91, P= 0.019). However, the significant association between 1349T>G polymorphism and breast cancer risk was not observed (P = 0.89). Larger studies are needed to confirm our results.
乳腺癌是世界上最常见的癌症，也是癌症相关死亡的主要原因之一，已成为一个重大的公共卫生问题。许多基因已被证明与乳腺癌的进展有关。人脱嘌呤/脱嘧啶内切酶1（APE1）是一种多功能蛋白质，在碱基切除修复（BER）途径中起重要作用。本研究的目的是评估伊朗北部人群中ApE1 -656T>G和1349T>G多态性与乳腺癌易感性之间的关联。从150例诊断为乳腺癌的患者和150例对照受试者中获取样本，并通过四引物扩增阻滞突变系统PCR（T-ARMS-PCR）进行基因分型。我们观察到患者和对照之间-656T>G多态性的基因型分布存在显著差异（P=0.03）。研究结果表明，具有变异TG基因型的个体患乳腺癌的风险显著降低（OR=0.55，95%CI=0.33 - 0.91，P=0.019）。然而，未观察到1349T>G多态性与乳腺癌风险之间的显著关联（P = 0.89）。需要更大规模的研究来证实我们的结果。
