Su Yongsheng, Liu Hui, Wang Jin, Lin Bojie, Miao Yong, Hu Zhiqi
Department of Burn and Plastic Surgery, The People's Hospital of Baoan Shenzhen Affiliated to Southern Medical University, Shenzhen, China.
Department of Plastic and Reconstructive Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, China.
Acta Histochem. 2015 Oct;117(8):798-802. doi: 10.1016/j.acthis.2015.07.005. Epub 2015 Aug 6.
Lysozyme is a well-known antimicrobial peptide that exists widely in mammalian skin and it is also expressed by pilosebaceous units. However, the exact location of lysozyme in hair follicles and whether it exerts any direct effects on hair follicle growth are unclear. To determine whether lysozyme affected hair growth in vitro, micro-dissected mouse vibrissae follicles (VFs) were treated in serum-free organ culture for 3 days with lysozyme (1-10μg/ml). After that, the effects of lysozyme on dermal papilla (DP) cells were also investigated. Lysozyme was mainly identified in DP and dermal sheath regions of VF by immunochemistry. In addition, 5-10μg/ml lysozyme had a promoting effect on shaft production. It was also associated with significant proliferation of matrix keratinocytes by immunofluorescence observation. Furthermore, lysozyme promoted hair growth by increasing the levels of alkaline phosphatase and lymphoid enhancer factor 1 in DP, as determined by Western blotting. These results indicate that lysozyme is a promoter of VF growth via enhancing the hair-inductive capacity of DP cells during organ culture.
溶菌酶是一种广为人知的抗菌肽，广泛存在于哺乳动物皮肤中，皮脂腺单位也可表达该物质。然而，溶菌酶在毛囊中的具体位置以及它是否对毛囊生长产生直接影响尚不清楚。为了确定溶菌酶在体外是否影响头发生长，将显微解剖的小鼠触须毛囊（VFs）在无血清器官培养中用溶菌酶（1-10μg/ml）处理3天。之后，还研究了溶菌酶对真皮乳头（DP）细胞的影响。通过免疫化学方法发现，溶菌酶主要存在于VF的DP和真皮鞘区域。此外，5-10μg/ml的溶菌酶对毛干生成有促进作用。通过免疫荧光观察发现，它还与基质角质形成细胞的显著增殖有关。此外，通过蛋白质免疫印迹法测定，溶菌酶通过提高DP中碱性磷酸酶和淋巴增强因子1的水平来促进头发生长。这些结果表明，在器官培养过程中，溶菌酶通过增强DP细胞的毛发诱导能力，从而成为VF生长的促进剂。
