Heterogeneity of anti-B monoclonal antibodies in reactivity to erythrocytes and saliva samples from various B secretor individuals.

In order to study the heterogeneity of anti-B monoclonal antibodies under the same conditions, twelve different types were produced from a mouse immunized with human blood group B erythrocytes, all of which were IgM antibodies that exhibited strong and specific agglutination activity for normal blood group B erythrocytes. However, analysis of the agglutination titer for blood group B2, B3, and rabbit erythrocytes revealed some qualitative differences among these antibodies. These differences were further clarified by comparing the inhibition pattern of the monoclonal antibody with saliva from B individuals, with that of ordinary human antiserum to show remarkable differences among individuals.