Sakata N, Kawakami N, Kuwaki Y, Kitahama M
Nihon Hoigaku Zasshi. 1989 Oct;43(5):430-7.
In order to study the heterogeneity of anti-B monoclonal antibodies under the same conditions, twelve different types were produced from a mouse immunized with human blood group B erythrocytes, all of which were IgM antibodies that exhibited strong and specific agglutination activity for normal blood group B erythrocytes. However, analysis of the agglutination titer for blood group B2, B3, and rabbit erythrocytes revealed some qualitative differences among these antibodies. These differences were further clarified by comparing the inhibition pattern of the monoclonal antibody with saliva from B individuals, with that of ordinary human antiserum to show remarkable differences among individuals.
为了在相同条件下研究抗B单克隆抗体的异质性，用人类B血型红细胞免疫小鼠制备了12种不同类型的抗体，它们均为IgM抗体，对正常B血型红细胞表现出强烈且特异性的凝集活性。然而，对B2、B3血型红细胞和兔红细胞的凝集效价分析显示，这些抗体之间存在一些质量上的差异。通过比较单克隆抗体与B个体唾液的抑制模式以及与普通人抗血清的抑制模式，进一步明确了这些差异，结果表明个体之间存在显著差异。
