Edelman L, Bach J F, Reviron J
Dev Biol Stand. 1984;57:43-7.
Monoclonal anti-A, anti-B, anti-H antibodies have been produced by a murine hybridoma. C57BL/6 mice have been immunized by different red blood cells. The main characteristics of these antibodies are the following: specificity: anti-A agglutination only A cells, including A2, A3, Ax (from a panel of 50 cells); anti-B agglutinates only B cells (B3, Bx, cis Ab and acquired B). Anti-H agglutinates all cells except H-deficient red cells. inhibition: it is only inhibited by the saliva of each corresponding secretor subject. immunofluorescence tests: it is able to detect A,B,H antigens on tissue sections, kidney, pancreas, liver. thermodynamics: the association constant is 2.10(7) 1/ml (for anti-A) and 3.10(8) for anti-B and anti-H. All are IgM. Monoclonal anti-N, and some anti-public antibodies such as anti-gerbich and anti-Rh 17 have been obtained too. All these antibodies could be useful as reagents and for the study of weak groups.
一种鼠杂交瘤产生了单克隆抗A、抗B、抗H抗体。C57BL/6小鼠已用不同的红细胞进行免疫。这些抗体的主要特性如下：特异性：抗A仅凝集A细胞，包括A2、A3、Ax（来自50个细胞的细胞系）；抗B仅凝集B细胞（B3、Bx、顺式Ab和获得性B）。抗H凝集除H缺陷红细胞外的所有细胞。抑制作用：仅被相应分泌型个体的唾液抑制。免疫荧光试验：能够在组织切片、肾脏、胰腺、肝脏上检测到A、B、H抗原。热力学：结合常数为抗A 2.10(7) 1/ml，抗B和抗H为3.10(8)。所有抗体均为IgM。还获得了单克隆抗N以及一些抗公共抗体，如抗杰比奇和抗Rh 17。所有这些抗体都可作为试剂用于弱血型组的研究。
