Monoclonal antibodies against blood group antigens.

Monoclonal anti-A, anti-B, anti-H antibodies have been produced by a murine hybridoma. C57BL/6 mice have been immunized by different red blood cells. The main characteristics of these antibodies are the following: specificity: anti-A agglutination only A cells, including A2, A3, Ax (from a panel of 50 cells); anti-B agglutinates only B cells (B3, Bx, cis Ab and acquired B). Anti-H agglutinates all cells except H-deficient red cells. inhibition: it is only inhibited by the saliva of each corresponding secretor subject. immunofluorescence tests: it is able to detect A,B,H antigens on tissue sections, kidney, pancreas, liver. thermodynamics: the association constant is 2.10(7) 1/ml (for anti-A) and 3.10(8) for anti-B and anti-H. All are IgM. Monoclonal anti-N, and some anti-public antibodies such as anti-gerbich and anti-Rh 17 have been obtained too. All these antibodies could be useful as reagents and for the study of weak groups.