Sharma Priyanka, Bajaj Bijender Kumar
School of Biotechnology, University of Jammu, New Campus, BAWE-WALI RAKH, Jammu 180006, India.
School of Biotechnology, University of Jammu, New Campus, BAWE-WALI RAKH, Jammu 180006, India.
Int J Biol Macromol. 2015 Nov;81:241-8. doi: 10.1016/j.ijbiomac.2015.08.008. Epub 2015 Aug 7.
Usage of renewable raw materials for production of fully degradable bioplastics (bacterial poly-3-hydroxybutyrate, PHB) has gained immense research impetus considering recalcitrant nature of petroleum based plastics, dwindling fossil fuel feed stocks, and associated green house gas emissions. However, high production cost of PHB is the major bottleneck for its wide range industrial applications. In current study, Bacillus cereus PS 10, a recent isolate, efficiently utilized molasses, an abundantly available by-product from sugar industries as sole carbon source for growth and PHB production. Most influential bioprocess variables i.e. molasses, pH and NH4Cl were identified based on Plackett-Burman-designed experiments. Design of experiment approach (response surface methodology) was further employed for optimization of these bioprocess variables, and an enhanced PHB yield (57.5%) was obtained. PHB produced by Bacillus cereus PS 10 was investigated using various physico-chemical approaches viz. thermogravimetric analysis, proton and carbon NMR ((1)H and (13)C) spectroscopy, melting point, elemental analysis and polarimetry for its detail characterization, and assessment for industrial application potential.
考虑到石油基塑料的难降解性、化石燃料原料的减少以及相关的温室气体排放，使用可再生原料生产完全可降解的生物塑料（细菌聚-3-羟基丁酸酯，PHB）已获得了巨大的研究动力。然而，PHB的高生产成本是其广泛工业应用的主要瓶颈。在当前的研究中，蜡样芽孢杆菌PS 10（一种最近分离出的菌株）有效地利用了糖蜜（制糖工业大量可得的副产品）作为唯一碳源用于生长和PHB生产。基于Plackett-Burman设计的实验确定了最具影响力的生物工艺变量，即糖蜜、pH值和氯化铵。进一步采用实验设计方法（响应面法）对这些生物工艺变量进行优化，获得了提高的PHB产量（57.5%）。使用各种物理化学方法对蜡样芽孢杆菌PS 10产生的PHB进行了研究，即热重分析、质子和碳核磁共振（（1）H和（13）C）光谱、熔点、元素分析和旋光测定，以对其进行详细表征，并评估其工业应用潜力。
