Applied Biotechnology Research Group, Department of Molecular and Applied Biosciences, School of Life Sciences, University of Westminster, London, UK.
Biotechnol J. 2012 Feb;7(2):293-303. doi: 10.1002/biot.201100122. Epub 2011 Dec 7.
The main hindrance in the use of polyhydroxyalkanoates (PHAs) as a replacement for existing petroleum-based plastics is their high production cost. The carbon source accounts for 50% of the cost for PHA production. Thus, increasing the yield and productivity of PHAs on cheap substrates is an important challenge for biotechnologists to support the commercialization and further applications of these polymers. In this study, we have investigated the use of an agricultural raw material, sugarcane molasses, as the main carbon source for poly(3-hydroxybutyrate) (P(3HB)) production by Bacillus cereus SPV. These studies were carried out in both shaken flasks and 2 L bioreactors. Various conditions were evaluated for their effects on biomass and P(3HB) accumulation. A high polymer yield was obtained, 61.07% dry cell weight (DCW) in a 1 L shaken flask study and 51.37% DCW in a 2 L fermenter study. These yields are 50% higher than previously observed with Bacillus cereus SPV. Hence, the results are encouraging and show that sugarcane molasses are a promising carbon source for an economical and commercially viable production of P(3HB).
聚羟基烷酸酯（PHAs）作为现有石油基塑料的替代品，其主要障碍是生产成本高。PHA 生产的碳源占生产成本的 50%。因此，提高廉价基质上 PHAs 的产量和生产力是生物技术人员面临的一项重要挑战，以支持这些聚合物的商业化和进一步应用。在这项研究中，我们研究了利用农业原料甘蔗蜜作为蜡状芽孢杆菌 SPV 生产聚（3-羟基丁酸酯）（P(3HB)）的主要碳源。这些研究在摇瓶和 2 L 生物反应器中进行。评估了各种条件对生物量和 P(3HB)积累的影响。在 1 L 摇瓶研究中获得了 61.07%的干细胞重量（DCW）的高聚合物产率，在 2 L 发酵罐研究中获得了 51.37%的 DCW。这些产率比以前用蜡状芽孢杆菌 SPV 观察到的提高了 50%。因此，结果令人鼓舞，表明甘蔗蜜是一种有前途的碳源，可以经济高效地生产 P(3HB)。
