Wang Yuan-long, Liu Zhen-min, Ren Jing, Guo Ben-heng
1 State Key Laboratory of Dairy Biotechnology , Ministry of Science & Technology, Shanghai, China .
2 Shanghai Engineering Research Center of Dairy Biotechnology , Shanghai, China .
Foodborne Pathog Dis. 2015 Aug;12(8):693-703. doi: 10.1089/fpd.2014.1916.
A precise and simplified method of sample preparation for the simultaneous quantification of the antibiotics β-lactam, macrolide, tetracycline, sulfonamide, and quinolone in bovine milk was developed. The central composite design of response surface methodology was used to design and optimize the method for the determination of six different antibiotic residues in milk. The recovery of each antibiotic was studied using a quick, easy, cheap, effective, rugged, and safe (QuEChERS) method. Octadecylsilane (C18), primary secondary amine (PSA), and sodium acetate (Na acetate) were the main factors affecting the recovery of each antibiotic. After optimization, the maximum predicted recovery rate was 84.18% for erythromycin under the optimized conditions of 101.20 mg C18, 52.00 mg PSA, and 1.01 g Na acetate. The recovery rates of the five other antibiotic residues ranged from 86.09% to 115.99%. The results suggested that modified QuEChERS could effectively be implemented in the analysis of antibiotic residues in milk.
开发了一种用于同时定量牛乳中抗生素β-内酰胺、大环内酯、四环素、磺胺类和喹诺酮类的精确且简化的样品制备方法。采用响应面法的中心复合设计来设计和优化测定牛奶中六种不同抗生素残留的方法。使用快速、简便、廉价、有效、耐用且安全（QuEChERS）的方法研究了每种抗生素的回收率。十八烷基硅烷（C18）、伯仲胺（PSA）和醋酸钠（醋酸钠）是影响每种抗生素回收率的主要因素。优化后，在101.20 mg C18、52.00 mg PSA和1.01 g醋酸钠的优化条件下，红霉素的最大预测回收率为84.18%。其他五种抗生素残留的回收率在86.09%至115.99%之间。结果表明，改良的QuEChERS可有效地用于牛奶中抗生素残留的分析。
