持续定向进化DNA结合蛋白以提高转录激活样效应因子核酸酶（TALEN）的特异性。
Continuous directed evolution of DNA-binding proteins to improve TALEN specificity.
作者信息
Hubbard Basil P, Badran Ahmed H, Zuris John A, Guilinger John P, Davis Kevin M, Chen Liwei, Tsai Shengdar Q, Sander Jeffry D, Joung J Keith, Liu David R
机构信息
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts, USA.
Molecular Pathology Unit, Massachusetts General Hospital, Charlestown, Massachusetts, USA.
出版信息
Nat Methods. 2015 Oct;12(10):939-42. doi: 10.1038/nmeth.3515. Epub 2015 Aug 10.
Nucleases containing programmable DNA-binding domains can alter the genomes of model organisms and have the potential to become human therapeutics. Here we present DNA-binding phage-assisted continuous evolution (DB-PACE) as a general approach for the laboratory evolution of DNA-binding activity and specificity. We used this system to generate transcription activator-like effectors nucleases (TALENs) with broadly improved DNA cleavage specificity, establishing DB-PACE as a versatile approach for improving the accuracy of genome-editing agents.
含有可编程DNA结合结构域的核酸酶可以改变模式生物的基因组，并有可能成为人类治疗药物。在此，我们提出了DNA结合噬菌体辅助连续进化（DB-PACE），作为一种用于DNA结合活性和特异性实验室进化的通用方法。我们使用该系统生成了具有广泛改善的DNA切割特异性的转录激活样效应物核酸酶（TALENs），确立了DB-PACE作为提高基因组编辑试剂准确性的通用方法。
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