Bi Guiqi, Liu Guoqiang, Zhao Ezi, Du Qingwei
a College of Marine Life Sciences, Ocean University of China , Qingdao , China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Jul;27(4):2554-6. doi: 10.3109/19401736.2015.1038801. Epub 2015 Aug 10.
454 GS FLX Titanium sequencing data were used to obtain the complete mitochondrial genome of Calliarthron tuberculosum (26,469 bp). The mitogenome contains 44 genes, including 2 ribosomal RNA, 19 transfer RNA, 5 ribosomal proteins, 1 ymf, 2 open reading frames (ORFs) and 16 genes involved in cellular respiration. The secY and rps12 are overlapped by 69 bp. rps3 and rpl16 also have a 45 bp overlapped region. Except for open reading frames near stem-loop region, the results show Calliarthron tuberculosum mtDNA has a high similarity with other Florideophyceae algae mitogenomes in gene synteny, structure and gene content. Phylogeny analysis indicates a close genetic relationship of Calliarthron tuberculosum with Sporolithon durum.
利用454 GS FLX Titanium测序数据获得了瘤状节石藻(Calliarthron tuberculosum)的完整线粒体基因组(26,469 bp)。该线粒体基因组包含44个基因,包括2个核糖体RNA、19个转运RNA、5个核糖体蛋白、1个ymf、2个开放阅读框(ORF)以及16个参与细胞呼吸的基因。secY和rps12重叠69 bp。rps3和rpl16也有一个45 bp的重叠区域。除了茎环区域附近的开放阅读框外,结果表明瘤状节石藻的线粒体DNA在基因同线性、结构和基因含量方面与其他红藻纲藻类的线粒体基因组具有高度相似性。系统发育分析表明瘤状节石藻与硬孢石藻(Sporolithon durum)有密切的亲缘关系。