Du Han, Zhu Liquan
Wei Sheng Wu Xue Bao. 2015 May 4;55(5):579-86.
To determine the function of high-affinity iron permeases, Ftr1 and Ftr2, we studied cell growth and filamentation ability of the ftr1/ftr1, ftr2/ftr2, and ftr1/ftr1 ftr2/ftr2 mutants under different culture conditions.
Cells of the wild type and mutants were cultured on different solid media at different temperatures. Cell growth and filamentation were observed.
Deletion of either one of the FTR genes had no effect on the growth under all conditions tested. Deletion of both FTR1 and FTR2 led to obvious growth defect on Spider media, although addition of FeCl3 restored their growth. The double mutant also grew much more slowly on nutrients-limited synthetic media such as Lee's glucose and Lee's GlcNAc (N-acetylglucosamine). Moreover, deletion of FTR1 enhanced filamentation, whereas deletion of FTR2 weakened this ability. Deletion of both FTR1 and FTR2 recovered the ability of filamentation.
Ftr1 and Ftr2 are very important for C. albicans growth under iron-limited condition and may participate in the utilization of some carbon sources including GlcNAc, ethnol, and glycerol. Ftr1 plays a negative, whereas Ftr2 plays a positive role in the regulation of filamentation in C. albicans.
为了确定高亲和力铁通透酶Ftr1和Ftr2的功能，我们研究了ftr1/ftr1、ftr2/ftr2和ftr1/ftr1 ftr2/ftr2突变体在不同培养条件下的细胞生长和丝状化能力。
将野生型和突变体的细胞在不同温度下培养于不同的固体培养基上。观察细胞生长和丝状化情况。
在所有测试条件下，缺失任何一个FTR基因对生长均无影响。缺失FTR1和FTR2两者导致在Spider培养基上出现明显的生长缺陷，不过添加FeCl3可恢复其生长。该双突变体在营养受限的合成培养基如李氏葡萄糖培养基和李氏N-乙酰葡糖胺（N-乙酰葡萄糖胺）培养基上生长也慢得多。此外，缺失FTR1增强丝状化，而缺失FTR2则削弱这种能力。缺失FTR1和FTR2两者可恢复丝状化能力。
Ftr1和Ftr2对于白念珠菌在铁限制条件下的生长非常重要，并且可能参与包括N-乙酰葡糖胺、乙醇和甘油在内的一些碳源的利用。Ftr1在白念珠菌丝状化调控中起负作用，则Ftr2起正作用。
