Jiang Xiaohong, Chen Yiping, Feng Guangyi, Luo Mingjie, Sun Qixiang, Li Chaoqian
Department of Geratic Respiratory Medicine, First Affiliated Hospital, Guangxi Medical University, Nanning 530021, China.
Department of Respiratory Medicine, First Affiliated Hospital, Guangxi Medical University, Nanning 530021, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2015 Aug;31(8):1067-70.
To investigate the number of T helper 9 (Th9) cells and the level of Th9 cell related cytokines in the lung tissues of mice with bronchial asthma and understand their relationship to the pathogenesis of bronchial asthma.
Sixteen SPF BALB/c mice were randomized into normal group and asthma group (8 mice in each group). After asthmatic models were established, the airway responsiveness was detected with non-invasive lung function machine, ELISA was used for determining the concentrations of cytokine interleukin-9 (IL-9), IL-10 and IL-13 in bronchoalveolar lavage fluid (BALF), HE staining and PAS staining was employed for observing the lung pathological changes, flow cytometry was used for testing the percentages of lung IL-9+ lymphocytes, IL-10+ lymphocytes and IL-13+ lymphocytes in lung CD3+ lymphocytes.
Compared with normal group, asthma group showed higher airway responsiveness, more severe airway inflammation and increased concentrations of cytokine IL-9 and IL-13, decreased concentration of cytokine IL-10 in BALF, higher percentages of lung IL-9+ lymphocytes and IL-13+ lymphocytes in CD3+ lymphocytes, and lower percentage of IL-10+ lymphocytes.
The expression of IL-9 was higher, but IL-10 was lower in asthma group. Th9 cells might play an important role in the pathogenesis of asthma.
探讨支气管哮喘小鼠肺组织中辅助性T细胞9（Th9）的数量及Th9细胞相关细胞因子水平，了解其与支气管哮喘发病机制的关系。
将16只SPF级BALB/c小鼠随机分为正常组和哮喘组，每组8只。建立哮喘模型后，用无创肺功能仪检测气道反应性，采用酶联免疫吸附测定法（ELISA）测定支气管肺泡灌洗液（BALF）中细胞因子白细胞介素-9（IL-9）、IL-10和IL-13的浓度，用苏木精-伊红（HE）染色和过碘酸雪夫（PAS）染色观察肺组织病理变化，用流式细胞术检测肺组织中CD3+淋巴细胞内IL-9+淋巴细胞、IL-10+淋巴细胞和IL-13+淋巴细胞的百分比。
与正常组相比，哮喘组气道反应性更高，气道炎症更严重，BALF中细胞因子IL-9和IL-13浓度升高，细胞因子IL-10浓度降低，CD3+淋巴细胞内肺组织IL-9+淋巴细胞和IL-13+淋巴细胞百分比升高，IL-10+淋巴细胞百分比降低。
哮喘组IL-9表达升高，IL-10表达降低。Th9细胞可能在哮喘发病机制中起重要作用。
