Tong Ruifang, Xu Liping, Liang Lihong, Huang Han, Wang Rui, Zhang Yinghui
Ward 1, Department of Respiration, Children's Hospital of Zhengzhou (Dongsan Street Hospital), Zhengdong, Zhengzhou 450053, P.R. China.
Children's Hospital of Zhengzhou, Zhengdong, Zhengzhou 450018, P.R. China.
Exp Ther Med. 2018 Mar;15(3):2480-2484. doi: 10.3892/etm.2018.5700. Epub 2018 Jan 4.
The purpose of the study was to detect the level of T-helper type 9 (Th9) cells and the cytokine interleukin-9 (IL-9) in peripheral blood of mice with bronchial asthma, and to explore the relationship between the expression of Th9 cells and the pathogenesis of asthma. Thirty female-specific pathogen-free (SPF) Bagg' albino (BALB)/c mice were selected and randomly divided into the control group (n=15) and the bronchial observation group (n=15). Mice in the bronchial observation group were treated with ovalbumin (OVA) for sensitization and induction of a mouse model of asthma. The airway reactivity of mice was measured by a mouse pulmonary function apparatus using the non-invasive pulmonary impedance method. The proportions of Th9 cells in peripheral blood of mice in the two groups were detected using flow cytometry. Digital polymerase chain reaction (dPCR), enzyme-linked immunosorbent assay (ELISA) and western blot analysis were applied to detect the levels of IL-9 messenger ribonucleic acid (mRNA) and proteins in peripheral blood and lung tissues of mice in the two groups, respectively. Compared with that in the control group, the expression level of Th9 cells in the peripheral blood of mice in the observation group was significantly elevated (P<0.05), the expression level of IL-9 proteins in the peripheral blood was significantly increased (P<0.05), and the levels of IL-9 mRNA and proteins in lung tissues were significantly increased (P<0.05). The results show that the levels of Th9 cells and their cytokine IL-9 in the peripheral blood of mice with bronchial asthma are significantly increased, suggesting that Th9 cells play important roles in the pathogenesis of asthma.
本研究旨在检测支气管哮喘小鼠外周血中9型辅助性T细胞（Th9）和细胞因子白细胞介素-9（IL-9）的水平，并探讨Th9细胞表达与哮喘发病机制之间的关系。选取30只雌性无特定病原体（SPF）的Bagg白化（BALB）/c小鼠，随机分为对照组（n = 15）和支气管观察组（n = 15）。支气管观察组小鼠用卵清蛋白（OVA）进行致敏和诱导建立哮喘小鼠模型。使用无创肺阻抗法通过小鼠肺功能仪测量小鼠的气道反应性。采用流式细胞术检测两组小鼠外周血中Th9细胞的比例。分别应用数字聚合酶链反应（dPCR）、酶联免疫吸附测定（ELISA）和蛋白质印迹分析检测两组小鼠外周血和肺组织中IL-9信使核糖核酸（mRNA）和蛋白质的水平。与对照组相比，观察组小鼠外周血中Th9细胞的表达水平显著升高（P < 0.05），外周血中IL-9蛋白的表达水平显著增加（P < 0.05），肺组织中IL-9 mRNA和蛋白质的水平显著增加（P < 0.05）。结果表明，支气管哮喘小鼠外周血中Th9细胞及其细胞因子IL-9水平显著升高，提示Th9细胞在哮喘发病机制中起重要作用。
