[癌相关成纤维细胞对胆囊癌细胞增殖和侵袭的影响]
[Effect of cancer-associated fibroblasts on proliferation and invasion of gallbladder carcinoma cells].
作者信息
Chen Chen, Shen Haoxin, Tao Jie, Song Huwei, Ma Li, Wang Lin, Geng Zhimin
机构信息
Department of Hepatobiliary Surgery, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China. E-mail:
出版信息
Nan Fang Yi Ke Da Xue Xue Bao. 2015 Aug;35(8):1149-54.
OBJECTIVE
To investigate the effect of cancer-associated fibroblasts (CAFs) on the growth and invasion of gallbladder cancer cells.
METHODS
The CAFs were isolated from human primary gallbladder carcinoma tissues by tissue culture and digestion methods. The cells were purified by differential adhesion method, and the primary cells were identified morphologically and immunocytochemically. The proliferation and invasion of two human gallbladder carcinoma cell lines (SBC-996 and GBC-SD) co-cultured with CAFs were detected by MTT and Transwell chamber assays.
RESULTS
Gallbladder carcinoma CAFs were isolated successfully by both tissue culture and enzyme digestion methods, and the latter method was more convenient and efficient. MTT and Transwell assays showed that CAFs significantly promoted the proliferation and invasion of the two gallbladder carcinoma cell lines.
CONCLUSION
CAFs can promote the proliferation and invasion of gallbladder carcinoma cells in vitro, suggesting the important role of CAFs in the development of gallbladder carcinoma.
目的
探讨癌相关成纤维细胞（CAFs）对胆囊癌细胞生长和侵袭的影响。
方法
采用组织培养和消化法从人原发性胆囊癌组织中分离CAFs。通过差速贴壁法纯化细胞，并对原代细胞进行形态学和免疫细胞化学鉴定。采用MTT法和Transwell小室法检测与CAFs共培养的两种人胆囊癌细胞系（SBC - 996和GBC - SD）的增殖和侵袭能力。
结果
通过组织培养和酶消化法均成功分离出胆囊癌CAFs，且酶消化法更简便高效。MTT法和Transwell法检测显示，CAFs显著促进了两种胆囊癌细胞系的增殖和侵袭。
结论
CAFs可在体外促进胆囊癌细胞的增殖和侵袭，提示CAFs在胆囊癌发生发展中具有重要作用。
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