Yang H R, Hu X P, Jiang C J, Qi J, Wu Y C, Li W, Zeng Y J, Li C F, Liu S X
College of Food Science and Technology, Hainan University, Haikou, China.
Lett Appl Microbiol. 2015 Nov;61(5):484-90. doi: 10.1111/lam.12483. Epub 2015 Sep 29.
About 1051 endophytic fungi were isolated from leaves, branches, barks and stems of Cephalotaxus hainanensis Li from four sites in Hainan, China. The fungi were identified as 21 genera by morphology and ITS sequences. One dominant species was Phomopsis quercella in Hainan Tropical Botanical Garden and Bawangling Nature Reserve, with relative frequency of 42·06 and 34·88% respectively. Another dominant species was Colletotrichum boninense in Wuzhishan and Jianfengling Nature Reserves, with relative frequency of 36·84 and 46·97% respectively. Among the selected 21 endophytic fungi, 17 strains (80·95%) had activity against at least one pathogenic bacteria, and 14 strains (66·67%) exhibited activity against at least one fungal pathogens. Neonectria macroconidialis showed strong inhibition against Staphylococcus aureus (inhibition zone being 20 mm), Bacillus subtilis (14 mm) and Streptococcus agalactiae (28 mm). Xylaria sp. showed strong inhibition against Escherichia coli (20 mm), Rhizoctonia solani (20 mm) and Sclerotinia sclerotiorum (17 mm). Verticillium bulbillosum showed great activity against Strep. agalactiae (32 mm) and Fusarium oxysporum (22 mm). These endophytic fungi showed potentials in medicine development.
Endophytic fungi from medicinal plants are an important source of novel and viable drugs. Cephalotaxus hainanensis Li is well known for leukaemia treatment and its endophytic fungi were isolated to investigate the diversity and antimicrobial activity. It was found that Ce. hainanensis Li had rich endophytic fungi, and some fungi showed strong antimicrobial activity against certain pathogens. These fungi can be used in medicine development.
从中国海南四个地点的海南粗榧的叶、枝、树皮和茎中分离出约1051株内生真菌。通过形态学和ITS序列将这些真菌鉴定为21个属。在海南热带植物园和霸王岭自然保护区，一个优势种是栎拟茎点霉，相对频率分别为42.06%和34.88%。在五指山和尖峰岭自然保护区，另一个优势种是香蕉炭疽病菌，相对频率分别为36.84%和46.97%。在所挑选的21株内生真菌中，17株（80.95%）对至少一种病原菌有活性，14株（66.67%）对至少一种真菌病原菌有活性。大孢新赤壳菌对金黄色葡萄球菌（抑菌圈为20毫米）、枯草芽孢杆菌（14毫米）和无乳链球菌（28毫米）有较强抑制作用。炭角菌属对大肠杆菌（20毫米）、立枯丝核菌（20毫米）和核盘菌（17毫米）有较强抑制作用。大孢轮枝菌对无乳链球菌（32毫米）和尖孢镰刀菌（22毫米）有很强的活性。这些内生真菌在药物开发方面具有潜力。
药用植物内生真菌是新型可行药物的重要来源。海南粗榧以治疗白血病而闻名，对其内生真菌进行分离以研究其多样性和抗菌活性。发现海南粗榧有丰富的内生真菌，一些真菌对某些病原菌有很强的抗菌活性。这些真菌可用于药物开发。
