Lin Xiaojing, Xu Hui, Liu Lan, Li Huixian, Gao Zhizeng
School of Marine Sciences, Sun Yat-sen University, Guangzhou, 510006 China.
Research Center of Chinese Herbal Resource Science and Engineering, Guangzhou University of Chinese Medicine, Guangzhou, 510006 China.
3 Biotech. 2020 Aug;10(8):346. doi: 10.1007/s13205-020-02345-8. Epub 2020 Jul 21.
In the current study, we report the high-quality draft genome sequence of sp. DH2, an endophytic fungus isolated from Prain in Tibet. The whole genome is about 45.8 Mbp, with a GC content of 53%. A total of 14,163 genes are predicted to encode proteins, and 557 of them are considered as unique, as no matches are found in five gene databases. A neighbor-joining phylogenetic tree based on internal transcribed spacer (ITS) region sequences shows that sp. DH2 was most closely related to . 47 biosynthetic gene clusters (BGC) were identified in sp. DH2 genome, and only 5 BGCs shows significant similarities to previously reported BGCs. The presence of 42 unique BGCs in sp. DH2 suggests that it has great potential to produce novel secondary metabolites.
在本研究中，我们报道了从西藏普氏原羚中分离出的内生真菌sp. DH2的高质量基因组草图序列。全基因组约为45.8 Mbp，GC含量为53%。共预测有14,163个基因编码蛋白质，其中557个被认为是独特的，因为在五个基因数据库中未找到匹配项。基于内部转录间隔区（ITS）区域序列构建的邻接系统发育树表明，sp. DH2与. 关系最为密切。在sp. DH2基因组中鉴定出47个生物合成基因簇（BGC），只有5个BGC与先前报道的BGC具有显著相似性。sp. DH2中存在42个独特的BGC，表明它具有产生新型次生代谢产物的巨大潜力。
