[紫背天葵多糖的分离、纯化与鉴定]

[Isolation, purification and identification of polysaccharides from Gynura divaricata].

作者信息

Wang Zhong-zhen, Zhang Qian-qian, Hao Xia, Lin Bing, Liu Zhi-hong, Song Hong-tao, Chen Lei

出版信息

Zhongguo Zhong Yao Za Zhi. 2015 Apr;40(8):1497-502.

Abstract

The purpose of this study was to isolate and purify polysaccharide from Gynura divaricata and analyze its monosaccharide composition. A water-soluble crude polysaccharide was obtained by hot water extraction, ethanol precipitation and deproteinization after degreasing. The crude polysaccharide then purified with DEAE-Sepharose Fast Flow column chromatography and dialysis. The monosaccharide composition and structure were analyzed by HPLC, UV spectrophotometer and 1H-NMR. The results showed that the purity and molecular weight of GDPS-2 and GDPS-3 were 87.3%, 2.03 x 10(4) Da and 90.9%, 4.29 x 10(4) Da, respectively. The UV spectrophotometer and 1H-NMR data suggested that glycosidic bond of GDPS-2 and GDPS-3 were a type. Both GDPs-2 and GDPs-3 were homogeneous polysaccharides, and GDPs-2 was mainly composed of glucuronic acid and xylose at a molar ratio of 1.1:0.63. GDPs-3 was mainly composed of rhamnose, glucuronic acid, galactose, xylose and galacturonic acid at a molar ratio of 0.32:6.0:0.21:1.75:4.3.

摘要

本研究的目的是从白凤菜中分离纯化多糖并分析其单糖组成。通过热水提取、乙醇沉淀以及脱脂后的脱蛋白处理获得了一种水溶性粗多糖。然后，该粗多糖经DEAE-琼脂糖快速流动柱色谱法和透析进行纯化。采用高效液相色谱法、紫外分光光度计和核磁共振氢谱对单糖组成和结构进行分析。结果表明，GDPS-2和GDPS-3的纯度分别为87.3%、分子量为2.03×10⁴ Da，以及纯度为90.9%、分子量为4.29×10⁴ Da。紫外分光光度计和核磁共振氢谱数据表明，GDPS-2和GDPS-3的糖苷键为α型。GDPS-2和GDPS-3均为均一多糖，GDPS-2主要由葡萄糖醛酸和木糖组成，摩尔比为1.1:0.63。GDPS-3主要由鼠李糖、葡萄糖醛酸、半乳糖、木糖和半乳糖醛酸组成，摩尔比为0.32:6.0:0.21:1.75:4.3。