[椭圆叶花锚水溶性多糖的分离、纯化及组成分析]
[Isolation, purification and composition analysis of water-soluble polysaccharide from Halenia elliptica].
作者信息
Liu Chun-Lan, Li Yang, Huang Xiao, Xi Gui-Yun, A Xi-Na
机构信息
College of Life and Environmental Science, MINZU University of China, Beijing 100081, China.
出版信息
Zhong Yao Cai. 2011 Aug;34(8):1219-23.
OBJECTIVE
To provide scientific cue for the use of Halenia elliptica.
METHODS
The crude polysaccharide was extracted from Halenia elliptica with hot water and precipitated by ethanol. The crude polysaccharide has been eliminated protein and fractionated by acidic ethanol. Four fractions HM1, HM2, HM3 and HM4 were got respectively.
RESULTS
By using DEAE-Sephadex A-25 gel filtration, HM1 was got. GC analysis indicated that HM1, HM2, HM3, HM4 and HM41 were composed of Rha, Ara, Xyl, Man, Gal and Glc. But their molar ratios were different.
CONCLUSION
Polysaccharide HM41 is isolated from Halenia elliptica for the first time.
目的
为椭圆叶花锚的应用提供科学依据。
方法
采用热水提取椭圆叶花锚粗多糖，乙醇沉淀，经脱蛋白、酸性乙醇分级，分别得到HM1、HM2、HM3和HM4四个级分。
结果
经DEAE - Sephadex A - 25凝胶过滤得到HM1。气相色谱分析表明，HM1、HM2、HM3、HM4和HM41均由鼠李糖、阿拉伯糖、木糖、甘露糖、半乳糖和葡萄糖组成，但它们的摩尔比不同。
结论
首次从椭圆叶花锚中分离得到多糖HM41。
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