Rajurkar V, Patil M, Shastri N V
Indian J Biochem Biophys. 1989 Oct;26(5):334-9.
Streptozotocin diabetic rats fed ad libitum exhibited hyperplasia of the small intestine. As compared to the control animals, the intestine of experimental animals grew in weight, length and total RNA and DNA contents. Intestinal cinnabarinate synthase activity in diabetic rats was however significantly lower. Developmental studies in albino rats indicated that, attainment of the terminal and highest activity of the enzyme tends to correspond with cessation of further increase in RNA and DNA contents of the intestine, thereby suggesting a possible relationship between cinnabarinate synthase and the hyperplastic changes observed. It was also observed that some properties of this enzyme, such as Km and Vmax are modified in diabetic condition. The enzyme was purified to apparent homogeneity and some of its kinetic and other properties were studied.
随意进食的链脲佐菌素诱导的糖尿病大鼠出现小肠增生。与对照动物相比，实验动物的肠道在重量、长度以及总RNA和DNA含量方面均有所增加。然而，糖尿病大鼠肠道中的朱砂合成酶活性显著降低。对白化大鼠的发育研究表明，该酶达到终末最高活性往往与肠道RNA和DNA含量的进一步增加停止相对应，从而提示朱砂合成酶与所观察到的增生性变化之间可能存在关联。还观察到该酶的一些特性，如米氏常数（Km）和最大反应速度（Vmax）在糖尿病状态下会发生改变。该酶被纯化至表观均一性，并对其一些动力学及其他特性进行了研究。
