Kheur Mohit, Harianawala Husain Hatim, Sethi Tania, Kheur Supriya, Acharya Aneesha, Mattheos Nikos
Department of Prosthodontics, M.A. Rangoonwala Dental College and Research Centre, Pune, Maharashtra, India.
Department of Oral Pathology, Dr D.Y. Patil Dental College and Hospital, Pune, Maharashtra, India.
J Investig Clin Dent. 2016 Nov;7(4):396-400. doi: 10.1111/jicd.12173. Epub 2015 Aug 18.
Human cytomegalovirus-1 (HCMV-1) and Epstein-Barr virus-1 (EBV-1) detection in submarginal plaque is linked to diseased states of the periodontium. In the present study, we evaluated the viral colonization of titanium and zirconia abutments by HCMV-1 and EBV-1 in a split-mouth study.
Forty dental implant abutments placed in 20 non-smokers were evaluated retrospectively. Each participant had received at least one each of titanium and zirconia abutments (in function for at least 1 year). HCMV-1 and EBV-1 were evaluated in these clinically-healthy peri-implant sites' submarginal plaque biofilm at one titanium and one zirconia abutment, one healthy tooth site, and serum using polymerase chain reaction assays. Related-samples McNemar test and Wilcoxon signed-rank test were used to determine the differences in viral detection frequency and load, respectively.
EBV-1 was detected at the titanium abutment in 60% of participants, but in none at their zirconia abutment (P = 0.04). HCMV-1 was detected at the titanium abutments in 90% of participants, and at the zirconia abutments in 70% of participants. This difference was not significant (P = 0.25). The differences in HCMV-1 viral load between the abutment types were insignificant (P = 0.075).
EBV-1 did not colonize the biofilm at the zirconia abutments as opposed to the titanium abutments in the same participants. Abutment material could contribute to differences in biofilm characteristics.
检测边缘下菌斑中的人巨细胞病毒1型（HCMV-1）和爱泼斯坦-巴尔病毒1型（EBV-1）与牙周组织的病变状态有关。在本研究中，我们通过一项分口研究评估了HCMV-1和EBV-1在钛和氧化锆基台上的病毒定植情况。
回顾性评估了20名不吸烟者口中植入的40个牙种植基台。每位参与者至少接受了一个钛基台和一个氧化锆基台（使用至少1年）。使用聚合酶链反应检测法对这些临床健康的种植体周围部位的边缘下菌斑生物膜中的HCMV-1和EBV-1进行评估，评估部位包括一个钛基台、一个氧化锆基台、一个健康牙齿部位以及血清。采用相关样本McNemar检验和Wilcoxon符号秩检验分别确定病毒检测频率和载量的差异。
60%的参与者在钛基台上检测到EBV-1，但在氧化锆基台上均未检测到（P = 0.04）。90%的参与者在钛基台上检测到HCMV-1，70%的参与者在氧化锆基台上检测到HCMV-1。这种差异不显著（P = 0.25）。不同基台类型之间HCMV-1病毒载量的差异不显著（P = 0.075）。
与同一参与者的钛基台不同，EBV-1未在氧化锆基台的生物膜中定植。基台材料可能导致生物膜特征的差异。
