Valli P, Zucca G, Botta L, Seghezzi R
Institute of General Physiology, University of Pavia, Italy.
J Auton Nerv Syst. 1989 Dec;28(3):211-7. doi: 10.1016/0165-1838(89)90148-3.
A method has been developed for the removal, preservation and electrophysiological study 'in vitro' of sympathetic lumbar chains (L1-L3) from subjects undergoing lumbar ganglionectomy in the treatment of peripheral vascular diseases. Extracellular recordings from interganglionic trunks, and intracellular recordings from single sympathetic neurons, were performed. The extracellular experiments substantiated the concept, hitherto deduced from animal experiments, that the preganglionic fibres in the sympathetic lumbar chain are mainly of a descending nature. In fact, stimulation of the interganglionic trunk cranial to ganglia is always much more effective in driving ganglion neurons to fire than stimulation of the interganglionic trunk caudal to ganglia. The intracellular experiments produced a good definition of the main electrical characteristics of human sympathetic neurons. The results can be summarized as follows: the resting membrane potential ranged from 50 to 75 mV (63.4 +/- 9.2 mV; 21 neurons); action potential amplitude from 62 to 93 mV (74.3 +/- 8.1 mV; 27 neurons); membrane input resistance was 42.3 +/- 12.6 m omega (8 neurons) and total membrane capacitance 83.7 +/- 15.3 pF (8 neurons).
已开发出一种方法，用于从接受腰神经节切除术治疗周围血管疾病的受试者身上取出、保存和进行体外电生理研究交感腰链（L1-L3）。进行了神经节间干的细胞外记录和单个交感神经元的细胞内记录。细胞外实验证实了迄今从动物实验推导出来的概念，即交感腰链中的节前纤维主要是下行性质的。事实上，刺激神经节上方的神经节间干在驱动神经节神经元放电方面总是比刺激神经节下方的神经节间干更有效。细胞内实验很好地定义了人类交感神经元的主要电特性。结果可总结如下：静息膜电位范围为50至75 mV（63.4±9.2 mV；21个神经元）；动作电位幅度为62至93 mV（74.3±8.1 mV；27个神经元）；膜输入电阻为42.3±12.6 mΩ（8个神经元），总膜电容为83.7±15.3 pF（8个神经元）。
