Beppu K
J Electron Microsc (Tokyo). 1989;38(6):430-40.
To study the localization of the progesterone and estradiol receptors by electron microscopy, the colloidal gold-labeled steroid hormones, intercalated with bovine serum albumin, were developed in the postembedding method. Among the tissues investigated in this study, receptors specifically distributed in the nuclei of the cells in the uterus and liver but not in the colon. Among the uterine smooth muscle cells in the estrus cycle, the frequency of the progesterone receptors in the nuclei indicated the highest level at a proestrus stage when the serum estradiol is maximum and the lowest level at a metestrus stage when the serum estradiol is minimum. On the other hand, the frequency of the estradiol receptor did not show significant changes among the stages of the estrus cycle. In the nuclei, the receptors specifically distributed on the euchromatin and on the peripheral regions of the heterochromatin, thought to be active regions of the transcription, and among the interchromatin granules. No receptors were observed within the nucleolus, the heterochromatin, the perinuclear chromatin, the nuclear sap, and the perichromatin granules. The colloidal gold-labeled steroids proved to be useful to study the microlocalization of the steroid hormone receptors in various target organs by electron microscopy.
为通过电子显微镜研究孕酮和雌二醇受体的定位，采用包埋后法制备了与牛血清白蛋白混合的胶体金标记甾体激素。在本研究中所调查的组织中，受体特异性分布于子宫和肝脏细胞的细胞核中，而在结肠中则未发现。在发情周期的子宫平滑肌细胞中，细胞核中孕酮受体的频率在动情前期血清雌二醇最高时显示出最高水平，而在动情后期血清雌二醇最低时显示出最低水平。另一方面，雌二醇受体的频率在发情周期各阶段未显示出显著变化。在细胞核中，受体特异性分布于常染色质以及被认为是转录活性区域的异染色质周边区域，以及染色质间颗粒之间。在核仁、异染色质、核周染色质、核液和染色质周颗粒内未观察到受体。胶体金标记的甾体激素被证明对于通过电子显微镜研究甾体激素受体在各种靶器官中的微观定位是有用的。
