Montero C N, Hefti F
Andrus Gerontology Center, University of Southern California, Los Angeles 90089.
Neurobiol Aging. 1989 Nov-Dec;10(6):739-43. doi: 10.1016/0197-4580(89)90011-0.
In young adult rats transection of the fimbria results in loss of cholinergic cell bodies in the septum and this lesion-induced loss is prevented by intraventricular administration of NGF. The present study examined whether NGF administration is equally effective in aging animals. Eighteen-month-old rats received fimbrial transections and were given intraventricular injections of NGF during four weeks. Septal cholinergic neurons were then visualized using NGF receptor immunohistochemistry, which represents a reliable marker for cholinergic neurons in the septal area. The fimbrial transections reduced the number of septal NGF receptor-positive cells to a similar extent as in young animals. NGF treatment of aging rats protected these cells as effectively as in young adult rats.
在年轻成年大鼠中，穹窿横断会导致隔区胆碱能细胞体丢失，而脑室内给予神经生长因子（NGF）可预防这种损伤诱导的细胞丢失。本研究检测了给予NGF在老龄动物中是否同样有效。18月龄大鼠接受穹窿横断，并在四周内给予脑室内注射NGF。然后使用NGF受体免疫组织化学法观察隔区胆碱能神经元，该方法是隔区胆碱能神经元的可靠标志物。穹窿横断使隔区NGF受体阳性细胞数量减少的程度与年轻动物相似。对老龄大鼠进行NGF治疗对这些细胞的保护效果与年轻成年大鼠相同。
