Tirumalasetti Jayasree, Patel Maulik, Shaikh Ubedulla, Harini K, Shankar J
Department of Pharmacology, Mamata Medical College, Khammam, Telangana, India.
Indian J Pharmacol. 2015 Jul-Aug;47(4):409-13. doi: 10.4103/0253-7613.161265.
Nerium oleander is traditionally used in various diseases because of its medicinal properties. One of its uses is in musculoskeletal disorder. The aim of the study was to evaluate the skeletal muscle relaxant activity of the aqueous extract of Nerium oleander flowers (AENOF) in albino rats in comparison with diazepam.
A total of 20 Swiss albino rats aged 6-7 weeks, of either sex, weighing about 100-150 g, were taken, and after acute toxicity studies two different doses were selected. The animals were divided into four different groups. The first group was kept as the control (normal saline), second as the standard (diazepam) and the remaining two groups as Test I and Test II, and given different doses of the AENOF. Skeletal muscle relaxant activity (motor coordination) on Rotarod and locomotor activity on photoactometer was performed. Statistical analysis was carried out by using analysis of variance, followed by Dunnett's multiple comparison tests.
The result from the Actophotometer test and Rotarod test showed that the extract of AENOF significantly reduced (P < 0.05) the motor coordination of the tested animals.
Our data indicates that AENOF possesses skeletal muscle relaxant activities.
夹竹桃因其药用特性而传统上用于多种疾病。其用途之一是治疗肌肉骨骼疾病。本研究的目的是比较夹竹桃花水提取物（AENOF）与地西泮对白化大鼠的骨骼肌松弛活性。
选取20只6 - 7周龄、体重约100 - 150 g的瑞士白化大鼠，雌雄不限，经急性毒性研究后选择两种不同剂量。将动物分为四个不同组。第一组作为对照组（生理盐水），第二组作为标准组（地西泮），其余两组作为试验I组和试验II组，并给予不同剂量的AENOF。进行了转棒试验中的骨骼肌松弛活性（运动协调性）和光电计上的自主活动试验。采用方差分析进行统计分析，随后进行Dunnett多重比较检验。
光电计试验和转棒试验结果表明，AENOF提取物显著降低了受试动物的运动协调性（P < 0.05）。
我们的数据表明AENOF具有骨骼肌松弛活性。
